If the HiVac pump is off - the system is not under vacuum to be able to run. The MS source and quad cannot be heated until the system is under vacuum.
The directions on how to vent and pump down the MS are in the Operating manual and also the Troubleshooting…
The graphite must deform to seal. It can only deform one time as there is no spring-back. Install it with the inlet cold and the inlet pressure set to zero psi. I like to put the liner in, then place the seal around it using a gloved hand, then tighten…
I have been working with Thermo Orbitrap Exploris 480.
Which type of Agilent Q-TOF MS could have similar performance compared to the Thermo LC-MS instrument?
Since i retired from the previous company for private reason, I am trying to find another…
Thanks Chris. as you are expected, the "Round Thing" was main cause for this issue. i clean both the sensor and Vaccum Fan. Since it was more than 10 year front panel never been inspected. now it running again normally. Againn.. Thanks for your advice…
Hello srlee ,
This would not be a tested or supported workflow. While GCMS SQ data can be processed, in limited testing it appears that if Profinder see ESI data it assumes it is accurate mass. Even attempting to open up the mass filters to something…
Hi! We have been experiencing issues with the sensitivity of our SpectrAA-50/55 using a nitrous oxide/acetylene flame to quantify vanadium. We have been using this setup fairly consistently to quantify vanadium in solution for ~2 years now and have not…
Hello,
So first of all, if the entries you need are numeric and per compound, I would use a CC constants file. There are ways to create one big constants file for all your compounds and use keys values to pull specific columns by method or other values…
This Information Applies To: 2200/4200/4250 TapeStation systems, TapeStation D1000 ScreenTape assay, TapeStation High Sensitivity D1000 ScreenTape assay, TapeStation analysis software
Issue:
A sharp peak appears at the lower end of the NGS library...
This Information Applies To: Agilent Variable Wavelength Detector G1314x and G7114A/B modules.
Issue
As UV deuterium lamps ages, the light intensity decreases, the sensitivity of detection may deteriorate. Replace the lamp when the intensity test...
This Information Applies To: Agilent 6850, 6890, 7820, 7890, 8850, 8860, 8890, Intuvo 9000 GC Systems with a Thermal Conductivity Detector (TCD)
Issue
The TCD temperature, column (carrier), makeup, and reference flow rates need to be optimized to...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue:
This article explains how to install the Agilent 2100 Expert software and connect a PC to an Agilent 2100 Bioanalyzer system.
Steps to follow:
The latest version of...
This Information Applies To: 2100 Bioanalyzer systems, 2100 Expert software
Issue: W hen trying to launch the Agilent 2100 Expert software, the process aborts and the user sees run-time error message :
Run-time error '50003 ' often indicates...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue
The DV 200 score is a quality score for evaluating quality of RNA derived from formalin-fixed paraffin-embedded (FFPE) samples established by Illumina Inc. in 2016. It represents...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software without Security Pack addon
Issue
The 2100 Expert software gives a counter mismatch error in the log book which may lead to data loss (blank lanes). Specifically...
This Information Applies To: Agilent ICP Expert software.
Issue
The software version is often asked and is a valuable information: to see if any updates are needed, if certain options are available etc.
Steps to Follow
Launch ICP Expert...
This Information Applies To: Agilent OpenLab CDS
Issue
This article provides step-by-step instructions to calculate peak-to-valley ratio in OpenLab CDS.
Background
The peak-to-valley ratio is calculated to indicate the quality of peak separation...
This Information Applies To: Agilent 6500 Series Q-TOF LC/MS systems, MassHunter Workstation 10.1 or below and MassHunter Workstation 11.0 .
Issue
Fully reset the Q-TOF LC/MS system via a power cycle without venting. For users who experience slow...