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  • -Collection of LC (HPLC) Resources Home
    • Collection of LC (HPLC) Resources
  • Best Practices for Using an Agilent LC System
  • Can the 1120 and 1220 Series LC Systems be Controlled by the Instant Pilot?
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  • Spectra are not showing for some peaks when collected only with Peak Apex
  • Use of Multiple Degasser Chambers for Isocratic and Binary Pumps Without a Solvent Selection Valve (SSV)
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Collection of LC (HPLC) Resources

Created by Roland Diaz-Bone Roland Diaz-Bone over 2 years ago | Last modified by kristen.sensabaugh kristen.sensabaugh 1 month ago

Here is a collection of Agilent LC Resources. Links to user manuals, firmware resources and maintenance videos, application resources,  Agilent InterAct! (Virtual LC Simulation), LC Supplies and Selector Tools and LC Troubleshooting Resources. Go to Collection of LC/MS Resources to view LC/MS information.

Are you awaiting the arrival of a new HPLC?  Resources for every stage of your new instrument journey are just a click away in Your InfinityLab HPLC Productivity Roadmap.  

LC User Manuals, Firmware Resources and Maintenance Videos

Resource

Description

LC User Manual Links

Collection of current LC hardware manuals

LC Firmware News and Downloads

CE Firmware News and Downloads

Download link to LC and CE Firmware and tools including Lab Advisor

Agilent 1100/1200/1290 Series Firmware Updates

Information Firmware Update

LC Firmware Background information

Background information for Firmware

HPLC Maintenance Videos

Step by step 1260 LC maintenance on YouTube

LC Application Resources

Resource

Description

LC Handbook

LC application primer

Solutions for Preparative HPLC - Application Compendium

Application compendium for preparative LC

Optimize binary pump

Optimization of LC and LC/MS

HPLC Separation Fundamentals

Basics of LC separation

New to HPLC Tips for Avoiding Beginner Pitfalls

Essentials for new LC users

LC Troubleshooting Series Retention Time Shifts

Best practice to troubleshoot retention time shifts

LC Column Troubleshooting – Isolating the Source of the Problem

Best practice to troubleshoot column related problems

Best practice fundamentals for using an LC-system

Best practice fundamentals for using an LC-system

Sample preparation fundamentals for chromatography

Handbook covering many different sample preparation technologies

Agilent LC Application finder

Application notes selection tool

Principles and Practical Aspects of Preparative Liquid Chromatography 

A primer for principles in preparative HPLC

Your reference guide to the Analysis of biopharmaceuticals and biomolecules

Agilent BioHPLC column selection guide

Method Transfer analytical HPLC to preparative HPLC

Application note for method transfer

Solutions for preparative HPLC

Application compendium

How to set up an LC method screening campaign with Method Scouting Wizard

Agilent Method Scouting Wizard Tutorial Series on YouTube

Agilent Interact (Virtual LC Simulation)

LC Supplies and Selection Tools

 Agilent Collection of Columns, Supplies, and Standards Resources

Resource

Description

InfinityLab LC Supplies Catalog

Consumables catalog

Agilent LC Capillary Finder

LC capillary search tool, more comprehensive overview on current LC capillary portfolio in LC supplies catalog.

Autosampler Vial Selector

Selection tool for vials, caps and septa fitting your application

Agilent LC Column and Sample Prep Navigator

Find the best column for your application

Syringe Filter Selector Tool

Find the best filter for your application

 LC Troubleshooting Resources

LC and LC/MS Troubleshooting | Agilent

LC Troubleshooting Series Videos | Agilent

LC Troubleshooting: Change in baseline profile | Agilent

LC Troubleshooting: Carry over | Agilent

LC Troubleshooting : Decrease in Retention Time | Agilent

LC Troubleshooting: Increase in LC Retention Time | Agilent

LC Troubleshooting : UV Detector Ignition Failed | Agilent

LC Troubleshooting : Split Peaks | Agilent

LC Troubleshooting: No Peaks During Run | Agilent

LC Troubleshooting : Unstable Pressure | Agilent

LC Troubleshooting: Peak Tailing | Agilent

LC Troubleshooting: Baseline spikes | Agilent

LC and LC/MS Troubleshooting

LC Troubleshooting Videos

  • LC Help
  • Selector Tools
  • CE Firmware
  • LC
  • HPLC
  • Lab Advisor
  • lc firmware
  • liquid chromatography
  • Liquid Chromatography User Manuals
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Anonymous
  • ISH
    ISH over 1 year ago in reply to sushans

    Hi,

    Better to call your local agilent agent to check this..May be vaccum pump of degasser is giving problem.

    So need service call..

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  • sushans
    sushans over 1 year ago

    How to fix the degasser issues it works for sometime in the beginning and then after 30-45 minutes the red light comes on and see the periodic pump pressure fluctuation. This persists even after prime the pump/purging the pump to remove any air bubbles trapped in the lines.

    Any suggestions for short term fixing of this problem with the degasser issues

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  • hplcuserx
    hplcuserx over 2 years ago

    Hi all, I find this post Preparative HPLC, semipreparative HPLC and analytical HPLC? -HPLC  in this page HPLC - All about HPLC  , I dont know, but reading the info I find really usefull, can you confirm if the info in this page its relevant, thanks you !

    Best regards

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  • lcdk
    lcdk over 4 years ago in reply to danielgalli

    Hello danielgalli,

     

    I check your question with our team and at the moment we don`t have a video available for this module. I want to share with you the user manual, where the needle replacement etc. is described with pictures  https://www.agilent.com/cs/library/usermanuals/public/G7129ABUser.pdf . As soon as we have new videos available, we will share them here in this space. In case you need support, please contact your regional customer contact center Contact Us | Agilent  or ask the community.

     

    Thanks,

    Daniel

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  • danielgalli
    danielgalli over 4 years ago

    Hello,

    Do you have any video about maintenance, needle replacement and needle seat of the vialsampler G7129x?
    Thanks and regards

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