• Created : 
  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Analytical LC Systems
  • Component :  LC Injectors and Sample Thermostats LC Valves
  • Product Name :  1100 Series LC System 1120 Compact LC 1200 Series LC System 1220 infinity II LC System 1260 Infinity II LC System 1260 Infinity III LC System 1260 Infinity LC System
  • Task :  Maintenance
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Cleaning or Changing the Rotor Seal of the LC Autosampler Injection Valve

Answer

This Information Applies To: Agilent LC Samplers 1100/1200/1260/1290 series: G1313A, G1329A/B, G1367A/B/C/D/E, G7129A/B/C, G4226A

Issue

The system presents high pressure due to a blockage in the injection valve. The valve does not switch from bypass to main pass. There are contaminants in the autosampler. Poor injection-volume reproducibility. Leaking injection valve.

Items Required

  • Wrench, 1/4 inch (supplied in HPLC Tool-Kit)
  • Hexagonal key, 9/64 inch (supplied in HPLC Tool-Kit)
  • Spare rotor seal according to sampler manual
  • Spare stator faces according to the sampler manual (If applicable)

Steps to Follow

  
Warning: Wear appropriate PPE (gloves, googles, and any other necessary personal protective equipment). While following the steps, you may come in contact with solvents while handling the instrument.

Opening the injection valve to access the rotor seal

  1. Switch off the module.
  2. Remove front cover.
  3. Take note of the capillary connection, ideally taking a picture of the configuration.
  4. Remove all capillary fittings from the injection-valve ports.
  5. Loosen each fixing bolt two turns at a time. Remove the bolts from the head carefully, not to drop any valve components.

Cleaning

  1. Remove any deposit visible in the rotor seal, part 4 in Figure 1, and further clean it by sonicating it for 5 minutes in methanol. 
    • Replace the part if it appears damaged or scratched.
  2. Verify that the Stator Head, item 2 in Figure 1, is free of residues, sonicate this part if needed.
  3. Reassemble the valve once the cleaning is completed.
  
Caution: Tighten them alternately with the 9/64'' wrench until the stator head is secure. The recommended torque for the screws is 2.25 Nm or until the handle of the 9/64'' hex key starts to twist in your hand.
Excessive tightening may impede the movement of the injection valve, triggering an error message. Resolve the error by untightening the bolts slightly.

                
The image shows a valve of a G1329A-B module, however the rotor seal is sufficiently similar to all the LC autosamplers 1100 - 1290 series.
Figure 1: The image shows a valve of a G1329A-B module, however the rotor seal is sufficiently similar to all the LC autosamplers 1100 to 1290 series.
1. Bolts, 2. Stator Head, 3. Stator ring, 4. Rotor seal, 5. Isolation seal

 
Learn how to effectively use your Agilent LC Autosampler:
Agilent LC samplers user manuals on Agilent.com

 

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