• Created : 
  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Analytical LC Systems
  • Component :  LC Pumps
  • Product Name :  1100 Series LC System 1200 Series LC System 1260 Infinity II LC System 1260 Infinity LC System
  • Task :  Maintenance
Related Articles

  • How to Troubleshoot no flow or no pressure issues: Binary/Quaternary/Isocratic Pumps - G131XX, and G711XX

    This Information Applies To: Agilent G1311A/B/C, G7111A/B, Quaternary Pumps, and G1310A/B, G7110B Isocratic Pumps, G1312A/B/C, G7112B Binary Pumps Issue: How to diagnose no flow or no pressure issues on binary, quaternary, or isocratic pumps. Resolution: The following information should help you find what part of the pump is causing a flow or pressure problem. Figure 1 and Figure 2 show the hydraulic paths of the pumps. Figure 1 - Hydraulic path for the Isocratic and quaternary pump...

  • How to Troubleshoot Low Pressure or No Flow/Solvent Coming Out of 1100/1200 Series Isocratic/Quaternary/Binary Pumps

    This Information Applies To: Agilent 1100/1200 Series G1310A Isocratic Pump, G1311A Quaternary Pump, and G1312A/B Binary Pump modules Issue This article shows different options to troubleshoot low pressure or no flow issues in 1100/1200 series Isocratic/Quaternary/Binary pumps. Items Required Open-end wrench 14 mm (p/n 8710–1924 ) Open-end wrench 1/4 - 5/16 inch (p/n 8710–0510 ) Pair of Tweezers Steps to follow The following steps will guide you to find out which part is causing...

  • No Pressure, No Flow, RT shift Issues: Cleaning Acetonitrile (ACN) Aggregations in Agilent Pumps

    This Information Applies To: Agilent LC pumps Issue: Stuck or obstructed check valves (Active Inlet Valve (AIV), Passive Inlet Valve (PIV), Outlet Ball Valve (OBV)) can cause issues such as no-flow or no-pressure, moderate to strong drops in pressure or retention time shifts. You can see the article How to Troubleshoot no flow or no pressure issues: Binary/Quaternary/Isocratic Pumps - G131XX, and G711XX to troubleshoot the issue. If one of the valves is the source of the blockage, and acetonitrile...

  • Modifying Solvent Compressibility for LC Pumps in OpenLab CDS

    This Information Applies To: Agilent OpenLab CDS, G7110B Isocratic Pump, G7111A/B, G5654A Quaternary Pumps, and G7112B Binary Pump. Issue Incorrectly applied solvent compressibilities can result in an increased pressure ripple, or inaccurate solvent composition in LC pumps. Background In HPLC, liquids under high pressures are pumped through the system, which can result in measurable liquid compression. Though the amount of compression is very small, it is enough to change the flow rate...

  • What Components determine Gradient Composition and Flow Accuracy in Binary and Quaternary Pumps

    This Information Applies To: Agilent Binary and Quaternary LC Pumps Issue When performing a gradient analysis, it’s important to know what component determines composition if it becomes necessary to troubleshoot chromatographic inconsistencies. Those inconsistencies can manifest themselves as peak retention time shifts (late or early peaks) and merged or missing peaks. Troubleshooting strategy depends on the pump type being used. This article will describe how binary and quaternary pumps create...

How to Change the Active Inlet Valve (AIV) and cartridge on a 1260 Isocratic, Binary or Quaternary Pump

Answer

This Information Applies To: Agilent Isocratic, Binary and Quaternary Pumps with AIV : G1310A/B,G1311A/B/C, G1312A/B, G7110B, G7111A/B

Issue

  • The inlet valve is defective (leaking) and causing backflow (shown by a flow/bubble going back and forth).
  • The leak test in Lab Advisor suggests the inlet valve may require replacement.
  • A large pressure fluctuation is observed (>2 bar)

 

Requirements

Switch off the pump and unplug the power cable. Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs to avoid liquid siphoning.

Items Required

Steps to Follow

Active Inlet Valve Parts Agilent
Figure 1. Parts of the AIV
 1. AIV cartridge, 2. AIV body

  1. Unplug the active inlet valve cable.

  2. Disconnect the solvent inlet tube from the inlet valve (be aware that solvent may leak out of the tube due to siphoning, so wear suitable gloves).

  3. Unscrew the adapter from the active inlet valve. 

  4. Using a 14 mm wrench loosen the active inlet valve nut and then remove the valve from the pump head. 

  5. Using a pair of tweezers remove the valve cartridge from the actuator assembly.

  6. Before inserting the new valve cartridge clean the area in the actuator assembly. Flush the cartridge area thoroughly with alcohol.

  7. Insert a new cartridge into the actuator assembly (make sure the valve cartridge is completely inserted into the actuator assembly).

     

      
    Note: The G1310A, G1311A and G1312A are 400 bar pumps, while G1310B, G1311B/C, G1312B, G7110B, G7111A/B are 600 bar pumps.

     

  8. Insert the new valve into the pump head. Using the 14 mm wrench turn the nut until it is hand tight.

  9. Position the valve such that the solvent inlet tube connection points towards the front and slightly to the right.

  10. Using the 14 mm wrench tighten the nut by while holding the valve into its final position. 

  11. Reconnect the adapter at the active inlet valve.

  12. Reconnect the solvent inlet tube to the adapter. Reconnect the active inlet valve cable to its connector.

  13. Purge the system with 30 mL of solvent in order to achieve a low-pressure ripple.

 

  Learn how to effectively maintain your Agilent valves:
Agilent 1260 Infinity LC with OpenLab CDS System Leak Errors 
Agilent 1260 Infinity LC with ChemStation System Leak Errors courses available from Agilent education
Was this helpful?
Privacy Statement
Terms of Use
Contact Us
Site Help