• Created : 
  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Automated Electrophoresis Systems
  • Component :  Femto Pulse Systems Fragment Analyzer Systems Oligo Pro II Systems ZAG DNA Analyzer Systems
  • Product Name :  5200 Fragment Analyzer System 5300 Fragment Analyzer System 5400 Fragment Analyzer System Femto Pulse System Oligo Pro II System ZAG DNA Analyzer system
  • Task :  Best Practices Operation
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Running Dye Labeled Samples on Agilent Parallel Capillary Electrophoresis Instruments

Answer

This Information Applies To: parallel capillary electrophoresis (CE) instruments, 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG DNA Analyzer, Oligo-Pro II

Issue:

How can a dye label affect the analysis with Agilent parallel CE instrument assays?

Resolution: 

Agilent has not validated the effect of dye labels in-house, so please proceed with caution. Theoretically, it is possible to run dye labeled samples (for example, labeled with dyes FAM, Cy5 etc.) on parallel CE instruments.

However, please be aware, due to the dye label, samples might migrate slightly differently which can affect sizing.

Also, depending upon the dye used, there can be effects on assay sensitivity. So overall data may not be fully accurate.

 

 Tip 
Learn how to effectively use Agilent parallel CE instruments : Fragment Analyzer User Manual, Femto Pulse User Manual, ZAG DNA Analyzer Manual, Oligo Pro II User Manual

 

For Research Use Only. Not for use in diagnostic procedures.

PR7000-8073

 

 
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