• Created : 
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  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Automated Electrophoresis Systems
  • Component :  Femto Pulse Systems Fragment Analyzer Systems Oligo Pro II Systems ZAG DNA Analyzer Systems
  • Product Name :  5200 Fragment Analyzer System 5300 Fragment Analyzer System 5400 Fragment Analyzer System Femto Pulse System Oligo Pro II System ZAG DNA Analyzer system
  • Task :  Maintenance Troubleshooting
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Full Power Cycle for Agilent Parallel Capillary Electrophoresis Instruments

Answer

This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, Oligo Pro II, ZAG, and their respective controller software

Issue:

A full power cycle can help to recover communication/connection issues of Agilent parallel capillary electrophoresis instruments (5200/5300/5400 Fragment Analyzer, Femto Pulse, Oligo Pro II, and ZAG systems). Communication and connection issues can materialize already during software start as connection failed error messages although the instrument is powered on and connected to the PC (see Figure1).

 
Figure 1. Examples of connection error messages

 Tip  Also the error Run aborted, Error moving syringe plunger might be resolved by a full power cycle. For more information, see here.

Steps to follow:  

  1. Exit any open programs (controller software, ProSize, etc.).

  2. Power off both the instrument and PC (see instrument power switch in Figure 2, A).



    Figure 2. Example back panel with instrument connections of the Fragment Analyzer instrument (A and B), and of the PC (C). All relevant parts for the full power cycle are marked.
    1. Labeled USB connections, 2. Fuse mount, 3. Power switch, 4. AC power connection, 5. Internet connection, 6. Computer monitor connection


  3. Unplug the instrument and PC power cables from the wall socket before unplugging the cables from the back of the instrument and PC (see AC power connections in Figure 2, A-C).

  4. Unplug the two USBs labeled CCD and HUB from the back of the PC (see Figure 2, C).

  5. Wait for 10 minutes.

  6. Reconnect USB cables to different, nonadjacent ports and reconnect power cables to the instrument and PC.

  7. Power on the PC and instrument and re-open the controller software.

 Tip  Learn how to effectively operate your Agilent parallel capillary electrophoresis system:
5200, 5300, and 5400 Fragment Analyzer System Manual
Femto Pulse User Manual
Oligo Pro II User Manual 
ZAG DNA Analyzer System Manual 

For Research Use Only. Not for use in diagnostic procedures.

PR7000-8120

 
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