Infinity 1260 II vialsampler standards dilution calibration programm

Hi,

there is no a downloadable program at the moment. But you could take a look at the Application Note Let Your Autosampler Do Your Pipetting (agilent.com). It describes the sampler programming approach to create the dilution series for calibration purposes.

Hi thanks for your response, but  there s no sequence example in this application note and description of the different functions in autosampler manual are very simple.
Is there any other manual?

regards.

L Stephan

If I understood correctly, you would like to use the mobile phase coming from the pump to perform the dilution. Unfortunately, it is not possible. The system is designed to protect the column from sudden pressure changes. Therefore, it cannot be in the main pass and be connected to the atmosphere (pre-column). All programmed valve switches (in injector program) are done with the needle in the needle seat.

So, glass inserts are the way to go in this case.

It depends on your workflow and how much you need to repeat these dilutions. There are automation solution like an Automated Liquid Handling from Agilent, Automated liquid handling, compact and flexible liquid handler | Agilent.

Cheers!

Thanks a lot forr all that.
I will test this :-)

Regards 

I Nadjan,
I m trying my firt dilution program but i ve got an error relative to the line "Draw maximum volume from air whith default speed".
I ve got this error


Do you have any idea about this error?
Thanks a lot.

L Stephan

Hi Lstephan, could you please copy here the numbers of the errors? Unfortunately, I could not recognize the error description on the screenshot.

Hi Nadjan, thanks for your response
A copy of the error :


Otherwise i m very suprised id received this response from Agilent



But the application note about dilution ( Let Your Autosampler Do Your Pipetting) is done with the same ALS than me ( loop 100µl and pump head 100µL) !

Thanks a lot

Hi Lstephan,

I am sorry to say, but the answer about the air bubble "sandwich" is incorrect. The air plug exists, but it does not affect the aspirated or injected volumes.

You mentioned earlier that you have a 100 µL loop. Could you please check if it is correctly configured? Right-click on the Sampler tile to get the menu and select Modify -> Flow Path

Are the sizes of the head and the loop configured as 100?

At the moment, this is the only thing that comes to my mind that could cause an error when you are trying to operate with 100 µL on the vialsampler. 

And the injector program is correct, you checked, right?  You are not trying to take/eject more than 100 µL?

Hi Lstephan,

I am sorry to say, but the answer about the air bubble "sandwich" is incorrect. The air plug exists, but it does not affect the aspirated or injected volumes.

You mentioned earlier that you have a 100 µL loop. Could you please check if it is correctly configured? Right-click on the Sampler tile to get the menu and select Modify -> Flow Path

Are the sizes of the head and the loop configured as 100?

At the moment, this is the only thing that comes to my mind that could cause an error when you are trying to operate with 100 µL on the vialsampler. 

And the injector program is correct, you checked, right?  You are not trying to take/eject more than 100 µL?

Hi Nadjan
Many thanks for your responses.

Yes i verified that analytical head and sample loop are 100µ volumes.

I send you a copy of different screen captures perharps will you see an error on my program?

erreurs.rtf

Thanks again

Hi,

did you try to set line number 5 in the injector program to the "Eject maximum volume"? On the screenshots, it was "Eject default volume". Does this help?

The last option would be to configure draw volumes 79 and 19 µL, as a trial. This would be something very new to me 

Hi,
Thanks i m not at the lab this week but i will try to change line 5.
I tried to modify both volumes in order to have a maximum added volumes less than 100µL ( like 10 from stock solution and 60 diluent volvent) but i had the same error.

I will tell you as soon as i ll test that.

Have a nice day.

Fingers crossed 

Hi Nadjan,

I continue my tests ;-)
So for the moment the injector program seems to work now. I weighed my vials several times and i ve got my 100µl as well in vial after dilution by five 80 + 20 µL. I saw the bubbles from the loop to shake my solutions.

In fact, the problem perharps comes from my sequence in wich i m injecting a blank from a vial in position P1-B1 as you can see here 


my injector program working :

Do you have an idea if there s a problem with my sequence test?

Thanks a lot.

L Stephan

Lstephan 

Are you still getting an error with this sequence?  What error message do you see?

/Andy