I really need to collect a chromatogram at 360 nm on my DAD. How do I do that?
What´s the issue? Just set the wavelength on your CDS and a suitable reference, if needed. If you share more details about the model#, I can point you to the user manual.
Here´s an old, but useful document about DAD optimization:
For the reference wavelength, there´s a guideline on page 12 of the DAD optimization document. There is no need to use a reference, but often it´s beneficial. Make sure the reference WL is well separated from the signal WL, as shown in the document. There are many pages on the topic "reference".
Thanks so much. It is a G1316A 1260 TCC. So I have to use a different
reference wavelength? Should it be lower or higher than 360 nm?
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Good, so then everything in the document applies to your DAD.
And here's the user manual for your detector:
I don't see why the tungsten lamp would be required at 400nm. You might have set a "Vis lamp required" somewhere in your CDS or one of your settings points to a higher WL.
Finding a suitable reference wavelength and reference bandwidth should be part of your method development. Please go over section "Sample and Reference Wavelength and Bandwidth" in the manual and see the pages for reference selection in the DAD document, I shared.
I am doing B9 and B12. So I picked 400nm as the ref wavelength for all my observed channels in order to observe 361nm.
If the ref wavelength chosen is also a major source of UV/Vis absorption in what is being studied, does it have an effect on the peak?