I really need to collect a chromatogram at 360 nm on my DAD. How do I do that?
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I really need to collect a chromatogram at 360 nm on my DAD. How do I do that?
Good, so then everything in the document applies to your DAD.
And here's the user manual for your detector:
https://www.agilent.com/cs/library/usermanuals/Public/G1315-90015_DAD-MWD-CD_USR_EN.pdf
Good, so then everything in the document applies to your DAD.
And here's the user manual for your detector:
https://www.agilent.com/cs/library/usermanuals/Public/G1315-90015_DAD-MWD-CD_USR_EN.pdf
I don't see why the tungsten lamp would be required at 400nm. You might have set a "Vis lamp required" somewhere in your CDS or one of your settings points to a higher WL.
Finding a suitable reference wavelength and reference bandwidth should be part of your method development. Please go over section "Sample and Reference Wavelength and Bandwidth" in the manual and see the pages for reference selection in the DAD document, I shared.
Thank you.
I am doing B9 and B12. So I picked 400nm as the ref wavelength for all my observed channels in order to observe 361nm.
If the ref wavelength chosen is also a major source of UV/Vis absorption in what is being studied, does it have an effect on the peak?
Also in order to use 400nm ref wavelength I had to turn on the visible lamp.