I really need to collect a chromatogram at 360 nm on my DAD. How do I do that?
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I really need to collect a chromatogram at 360 nm on my DAD. How do I do that?
What´s the issue? Just set the wavelength on your CDS and a suitable reference, if needed. If you share more details about the model#, I can point you to the user manual.
Here´s an old, but useful document about DAD optimization:
Thanks so much. It is a G1316A 1260 TCC. So I have to use a different
reference wavelength? Should it be lower or higher than 360 nm?
Best Regards,
Tony
Tony Montanari, Ph.D.
Quality Services Manager
Earth's Creation, USA, Inc.
18 Page Court
Travelers Rest, SC 29690
864-610-6000 x205
864-616-7087 cell phone talk/text
Honestly, I doubt it is a G1316A - that would be a column thermostat, which can only heat up or cool down columns.
For the reference wavelength, there´s a guideline on page 12 of the DAD optimization document. There is no need to use a reference, but often it´s beneficial. Make sure the reference WL is well separated from the signal WL, as shown in the document. There are many pages on the topic "reference".
For the reference wavelength, there´s a guideline on page 12 of the DAD optimization document. There is no need to use a reference, but often it´s beneficial. Make sure the reference WL is well separated from the signal WL, as shown in the document. There are many pages on the topic "reference".