Hi Kenforce,
In this case, I would run the pressure decay test, as you could have a small leak that is causing the inconsistency. I would also consider increasing your oven equilibration time by a 0.5min or so, just to ensure the column phase is at…
Hi Marty,
We have a FID and an MS connected to the same system. We only want to acquire the signal of FID, but if we want to report the method parameters we are “forced” to acquire both (we do not really know why and we have already ask the question…
Sorry for the delayed response. I've found that either works fine. I typically try to keep injection volume low (0.2-1 uL for 1uM protein). I've mostly been using 50mM HEPES 150mM NaCl and get great deconvolution results, even with a 3 min short method…
I am getting message "draw command failed, EE34205" while running batch in G7129B infinity 2 autosampler. I have tried replacing transport assembly, but error is same. I have used another module G7129A Infinity autosampler with this LC, but same error…
How do you set up an auditory sound alert for when a method run completes? I was thinking it may have been a runtime event but I I can’t see my to find it .I set a alarm on my phone for the length of the experiment to remind myself to go check and set…
Lines 3-5 (blank, blank, 1/100ppb) make me think there is a contamination issue. Check the cps of Ni on those first 4 blanks. If you see a downward trend, that could be an indication of a Nickel contamination somewhere in the instrument that is washing…
For Research Use Only. Not for use in diagnostic procedures.
Here is a "best of" collection of links to manuals and tools for Agilent Automated Electrophoresis products (Bioanalyzer, TapeStation, Fragment Analyzer, ZAG DNA Analyzer, Femto Pulse, Oligo…
Hello,
My name is Ben Niemi from the New Hampshire Public Health Laboratories. We are currently running 6470 QQQ LC-MS/MS with a 1260 Infinity II LC Stack.
Recently we've encountered some condensation issues in our column compartment on the line that…
This Information Applies To: Agilent 5975 and 5977 series GC/MSD
Issue:
Tuning the Agilent GC/MSD with EI sources, requires the use of perfluorotributylamine (PFTBA). The calibration vial will be depleted eventually due to regular use. This article...
This Information Applies To: Agilent 5975 and 5977 series GC/MSD
Issue:
If the PFDTD signal is absent or low, but the reagent ions are normal, you may have run out of CI Calibrant.
Inspect the calibration vial on the back of the flow controller...
This Information Applies To: Agilent 5975 and 5977 Series GC/MSD
Issue:
For some GC/MSD maintenance or repair asks, it is required to open the side plate (filament exchange, ion source cleaning, electron multiplier exchange, column installation...
This Information Applies To: Agilent GC/MS MassHunter Acquisition Software Agilent 5975/5977 GC/MSD
Issue
Use the Early Maintenance Feedback (EMF) tool to track the GC/MSD's consumable resource and part use, and to configure alerts for when they...
This Information Applies To: Agilent GC/MSD systems
Issue:
After a filament fails, there will be a system error message: "No emission current". Switching to the other filament (except for the CI ion source, which has just one filament), and then...
This Information Applies To: Agilent 7693A automatic liquid sampler tray
Issue:
The orange Align Mode light comes on . T he sample tray of the Agilent automatic liquid sampler 7693A will not operate again until the alignment procedure has successfully...
This Information Applies To: Agilent 7693A Automatic Liquid Sampler
Issue:
The automatic liquid sampler may need to be replaced, in case it is worn or dirty.
Steps to follow
To remove the syringe:
Open the injector door.
Slide...
This Information Applies To: Agilent 7693A Automatic Liquid Sampler
Issue:
Two turrets are provided with the 7693A injector, the 16-sample standalone turret, and the 3-sample transfer turret used with the 150-sample tray (See Figure 1 ). The turret...
This Information Applies To: Agilent 7697A Headspace Sampler
Issue:
There is a potential leak external to the Headspace unit that can cause loss of pressure.
Steps to Follow:
This article is a troubleshooting guide for when you suspect there...
This Information Applies To: Agilent 7693A Automatic Liquid Sampler
Issue:
This document explains how to replace the syringe carrier assembly in case you want to change from standard to high volume syringes, or if an Agilent support representative...