The file is a pre-set qPCR program to run a plate filled with water used to test the basic raw fluorescence signal of AriaMx Optical modules.
File required for Performing a Background Calibration
The GC cycle time should be the time from injection until the GC is ready to run again. This includes the analysis run, cool down, and equilibration. Based on the times you listed, it would be roughly 40 minutes. It may be worth timing to make sure. The…
Hi howard_sanford, my apologies for a slow response, but thank you very much for the help and advice. I was able to get the filter for Cal and CC to work, and I appreciate the discussion of my larger problem. I think I'm probably several (many) steps…
Hello, thank you for your help. I had tried loading different processing methods but I didn't realize the results were not linked to any method. It turns out the result files were read-only and could not be linked to any method. I moved the result files…
The peaks look similar, essentially the same sensitivity. I'd look at the background on your older runs and the background now. It could be that most of the previous background was one, two, a few, ions and they're gone now. Was that small but still noticeable…
That is one failure mode of Platinum Resistance Thermometers. It can happen one degree to the next higher degree. The heater/sensor must be replaced, part number G1099-60107.
See also: (+) AUX 2 heater fault - Forum - GC/MS - Agilent Community
This Information Applies To: Agilent GC Systems
Issue
Excessive baseline noise can be introduced at different points including sample preparation, sample introduction, separation, and the detector. The excessive background can cause reduced signal...
This Information Applies To: Agilent GC Systems
Issue
Baseline instability that is not reproducible across multiple runs can be introduced at different points including sample introduction, separation, and the detector. The baseline instability...
This Information Applies To: Agilent GC Systems
Issue
Peak fronting is present in a chromatography peak when it has an excessive asymmetry with a leading edge. A normal peak is almost symmetrical.
Other chromatographic problems are identified...
This Information Applies To: Agilent GC Systems
Issue
A shift in retention time can occur for single, multiple or all peaks.
Other chromatographic problems are identified in Basic Troubleshooting for GC Systems . Regular maintenance of your instrument...
This Information Applies To: Agilent GC Systems
Issue
A change in peak size can occur for single, multiple or all peaks.
Other chromatographic problems are identified in Basic Troubleshooting for GC Systems . Regular maintenance of your instrument...
This Information Applies To: Agilent GC Systems
Issue
Peak splitting is when a single compound is shown in the chromatogram as two peaks usually unresolved, or one of the peaks is a shoulder.
Other chromatographic problems are identified in Basic...
This Information Applies To: Agilent GC Systems
Issue
This test isolates the point of contamination to the sample introduction system, which includes the gas supply, gas supply lines, and the sample inlet. It does not include the syringe or autosampler...
This Information Applies To: Agilent GC systems
Issue
Peak tailing is present in a chromatography peak when it has an excessive asymmetry with a trailing edge. A normal peak is almost symmetrical.
Other chromatographic problems are identified...
T his Information Applies To: Agilent 8890/8860/7890/7820/6850/6890 GC Systems with TCD (Thermal Conductivity Detector)
Issue
Problems observed with thermal conductivity detector (TCD) results can have different underlying causes.
Resolution...
This Information Applies To: All GCs, 6890, 6850, 7890, 7820, 8890/8860, Intuvo 9000
Issue:
This article is a troubleshooting guide for when the Flame Ionization Detector (FID) does not ignite or the flame keeps going out.
Cause:
If the FID...