Sorry for the delayed response. I've found that either works fine. I typically try to keep injection volume low (0.2-1 uL for 1uM protein). I've mostly been using 50mM HEPES 150mM NaCl and get great deconvolution results, even with a 3 min short method…
I am getting message "draw command failed, EE34205" while running batch in G7129B infinity 2 autosampler. I have tried replacing transport assembly, but error is same. I have used another module G7129A Infinity autosampler with this LC, but same error…
Hello all! We are currently familiarising ourselves with the MassHunter data aquisition software (LC/MS Data Aquisition for 6200 series TOF/6500 series Q-TOF. Version B.08.00 Build 8.00.8058.3 SP1). We have been trying to find the most efficient way of…
How do you set up an auditory sound alert for when a method run completes? I was thinking it may have been a runtime event but I I can’t see my to find it .I set a alarm on my phone for the length of the experiment to remind myself to go check and set…
Lines 3-5 (blank, blank, 1/100ppb) make me think there is a contamination issue. Check the cps of Ni on those first 4 blanks. If you see a downward trend, that could be an indication of a Nickel contamination somewhere in the instrument that is washing…
For Research Use Only. Not for use in diagnostic procedures.
Here is a "best of" collection of links to manuals and tools for Agilent Automated Electrophoresis products (Bioanalyzer, TapeStation, Fragment Analyzer, ZAG DNA Analyzer, Femto Pulse, Oligo…
Hello,
My name is Ben Niemi from the New Hampshire Public Health Laboratories. We are currently running 6470 QQQ LC-MS/MS with a 1260 Infinity II LC Stack.
Recently we've encountered some condensation issues in our column compartment on the line that…
Recommendations:
Increase the Oven Temperature equilibration time from 0.5 minutes to 1 to 1.5 minutes. The GC oven walls need more time to cool down from 300° C back to 50° C to be ready for the next run. This affects run-to-run reproducibility.
…
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
The torch, bonnet, and platinum shield plate must be installed after performing maintenance, or replacing with new components.
Items Required
Standard...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
The torch, bonnet, and shield plate must be removed and inspected during routine maintenance, and when troubleshooting plasma ignition problems. Faults with...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
This procedure tests for an air leak in the sample introduction system, or fault with spray chamber draining, by sealing the end of the torch with a piece...
This Information Applies To: 7700, 7800, 7850, 7900 Agilent ICP-MS instruments
Issue
Error messages related to gas flows can cause the plasma to shut off or disable plasma ignition.
The troubleshooting procedure in this article is applicable for...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
Error messages related to water flow or water valve issues can cause the plasma to shut off or disable plasma ignition. While a warning message will still...
This Information Applies To: Agilent Fluorescence Detectors (FLD)
Issue
Sometimes it’s necessary to change FLD EX and EM wavelengths during a run since not all components will excite or emit at the same wavelengths. However, when the wavelength...
This Information Applies To: Agilent Analytical Scale LC Systems
Issue
To troubleshoot high backpressure within an HPLC system, it’s necessary to understand the expected pressure throughout an operating system. The total pressure for an LC is measured...
This Information Applies To: Agilent LC Samplers except dual needle multisamplers
Issue
High pressure in the LC sampler is sometimes due to a clog in the needle seat assembly, the needle, or the sample loop. The needle can pick up sample particulates...
This Information Applies To: All OpenLab CDS ChemStation Edition software-Standalone Workstation
Issue
After launching OpenLab ChemStation Online, the following error is shown: “ChemStation ChemMain has stopped working” . OpenLab ChemStation Online...
This Information Applies To: Agilent AriaMx, Software version 1.8 and later
Issue
The Aria Warm up Feature was introduced with Aria SW/FW 1.8 to reduce baseline drift. The warm-up feature is used to speed up the heating process that would normally...