Hi AnniarysRnD,
Check in your Processing Method>> System Suitability>>Separation performance computation>> Void time (min). In order to calculate column performance parameters, including Peak_CapacityFactor or Capacity factor of k', you need to enter…
I had notice that the air volume is set to 1ul which is the same as the sample volume (I’m assuming is the same as the injected volume). You could try to set the air volume to 0ul and observe if the injection is consistent after this.
Hi Susana,
Thank you for contacting Cell Analysis Technical Support. Unfortunately, the BIoTek part of our team is not yet on the Agilent Community. However, they can be reached at the below options:
Phone Contact: 800-227-9770 (Option 3, option…
Hi Mike, Thank you, yes correct, here is our situation to clarify, we upgraded in order to retire the Win7 OS. We had methods on Agilent UV 8454 ChemStation B.05.02 that managers would create, reviewers would approve, and analyst could use the methods…
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Elemental analysis of sodium ion battery cathode materials by Agilent ICP-OES by ICP-MS on March…
Wow, your peaks are super close to each other! Anything minor will affect your chromatogram. Maybe you should search for a better method? Anyways, what impacts the most are flow (are you running a gradient method?), organic concentration (again, gradient…
You can calculate the pressure on the flow cell by disconnecting the inlet capillary in the VWD. So the pressure in the VWD is the system pressure MINUS the pressure without VWD
This Information Applies To: Agilent Liquid Chromatography (LC) systems
Issue:
Shutting down the HPLC for a long period requires following this general procedure to prevent damage to the system and the column.
Steps to follow:
Store the...
This Information Applies To: Agilent G7129A/B Autosampler with Sample Thermostat/Cooler G7167-60101 or G7167-60005.
Issue:
The autosampler may stop working from time to time, potentially giving off loud noises and switching off the light. The only...
This Information Applies To: Agilent 5977A/B GC/MSD with EI source, EI Extractor Source, or CI Source.
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte...
This Information Applies To: Agilent HES source, Agilent 5977 Series GC/MSD
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte response not improving...
This Information Applies To: Agilent 5977 and 5975 Series GC/MSD systems
Issue
The autotune fails and reports the error Tune action stopped: Gain and offset = 0 and peaks are too narrow!
Resolution
The MSD autotune program is run after routine...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
Condition new or cleaned ICP-MS skimmer cones and sampler cones by aspirating a Conditioning Solution before use.
Background
Drift up can be a sign of...
Turn On the GC/MS Interface and Oven Using OpenLab CDS
Multiple Results
This Information Applies To: Agilent GC/MS instruments controlled by OpenLab CDS software
Issue
Turning on the GC/MS interface heater and Oven using OpenLab CDS software.
Steps to Follow
From the Home tab, click Method > Instrument Setup...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900)
Issue
The signal monitor function in MassHunter can help with troubleshooting low sensitivity, carry over, contamination, or high background. It can also be used to optimize...
This Information Applies To: Agilent UV detectors (Variable Wavelength Detectors, Multiple Wavelength Detectors, and Diode Array Detectors)
Issue
Checking accumulated hours on the UV lamp.
Steps to Follow
There are four ways to check the accumulated...
This Information Applies To: Agilent 7700/7800/7850/7900/8800/8900 ICP-MS
Issue:
Poor internal standard (ISTD) mixing can cause high relative standard deviation (RSD).
The recommendations in this article are applicable if:
It takes a long...