At 1 mL/min pumping water, the pressures are as follows:
Pump + Column = 53 bar
Pump + Column + 0.1 mm ID 30 cm tubing = 70 bar
Pump + Column + Tubing + Flow Cell = 75 bar
I have also confirmed that this pressure fluctuation has nothing to do…
It all depends on your following steps. You can even use Methanol or Acetonitrile for the protein precipition: Protein Precipitation for Biological Fluid Samples Using Captiva EMR-Lipid
See also the chapter on protein precipitation in Agilent's Handbook…
The Digest of upcoming webinars has been updated as of April 30.
Did you miss April's GC Live Session Recording and Discussion Thread? Check it out here: ( +) Diagnostics From Your Desk, Troubleshooting From Your Kitchen Table - Follow-up Discussion…
Hello cjwwfd ,
B.06.00 is out of support at this point, but I can offer some general suggestions for current revisions and hopefully you can apply them to your version. Version 12.0 is the current release. If possible, I would investigate your options…
I'm experimenting the same error. Logbook displays <MSQQQ_1: QqQ fault detected: 1.23 vacuum system is not ready. This is typical during a pump down cycle or when the high vacuum pump is turned off during a vent cycle or fault condition>. Does anyone…
Hello DET ,
Sorry for the late response on this.
What sort of spiking do you see usually? What is the output of the FID, in pA?
Are you using any UPS systems, or are the GCs plugged directly into the wall?
Hi, I think it would be a good idea to upload your startup tune report in order to help you better.
Also I think you could take advantage of reading this post: https://community.agilent.com/technical/atomic-spec/f/forum/470/icp-ms-manual-tuning
Hello,
So, file based workstations store the full path to the methods in the sequences. If there is any change between the systems in the project path this is the expected behavior if you copy a sequence. If you open the sequence, browse out and find…
Hello guys,
I recently discovered, that it is possible to change your font properties in the injection list via shortcut (ctrl + b for "bold" and so on..).
Therefore I wanted to ask you, if there is some code for changing font types for CCF outputs…
This Information Applies To: Agilent Liquid Chromatography (LC) systems
Issue:
Shutting down the HPLC for a long period requires following this general procedure to prevent damage to the system and the column.
Steps to follow:
Store the...
This Information Applies To: Agilent G7129A/B Autosampler with Sample Thermostat/Cooler G7167-60101 or G7167-60005.
Issue:
The autosampler may stop working from time to time, potentially giving off loud noises and switching off the light. The only...
This Information Applies To: Agilent 5977A/B GC/MSD with EI source, EI Extractor Source, or CI Source.
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte...
This Information Applies To: Agilent HES source, Agilent 5977 Series GC/MSD
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte response not improving...
This Information Applies To: Agilent 5977 and 5975 Series GC/MSD systems
Issue
The autotune fails and reports the error Tune action stopped: Gain and offset = 0 and peaks are too narrow!
Resolution
The MSD autotune program is run after routine...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
Condition new or cleaned ICP-MS skimmer cones and sampler cones by aspirating a Conditioning Solution before use.
Background
Drift up can be a sign of...
Turn On the GC/MS Interface and Oven Using OpenLab CDS
Multiple Results
This Information Applies To: Agilent GC/MS instruments controlled by OpenLab CDS software
Issue
Turning on the GC/MS interface heater and Oven using OpenLab CDS software.
Steps to Follow
From the Home tab, click Method > Instrument Setup...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900)
Issue
The signal monitor function in MassHunter can help with troubleshooting low sensitivity, carry over, contamination, or high background. It can also be used to optimize...
This Information Applies To: Agilent UV detectors (Variable Wavelength Detectors, Multiple Wavelength Detectors, and Diode Array Detectors)
Issue
Checking accumulated hours on the UV lamp.
Steps to Follow
There are four ways to check the accumulated...
This Information Applies To: Agilent 7700/7800/7850/7900/8800/8900 ICP-MS
Issue:
Poor internal standard (ISTD) mixing can cause high relative standard deviation (RSD).
The recommendations in this article are applicable if:
It takes a long...