Agilent Community
Agilent Community
  • User
  • Site
  • Search the Community
  • User
  • Home
  • Technical Areas
    Technical Areas
    • AssayMap
    • Atomic Spectroscopy
    • Automated Electrophoresis
    • Cell Analysis
    • Consumables
    • CrossLab Connect
      CrossLab Connect
      • Service Management
      • Smart Alerts
    • Dissolution
    • GC
    • GC/MS
    • iLab Operations Software
    • LC
    • LC/MS
    • Life Sciences Reagents
    • Molecular Spectroscopy
      Molecular Spectroscopy
      • Molecular Spectroscopy - UV-Vis & UV-Vis-NIR, FTIR, Raman & Fluorescence
      • 8700 LDIR User Group
      • Transmission Raman User Group
    • Real-Time PCR (qPCR)
    • Sample Preparation
    • Software
      Software
      • Chromatography Software
        OpenLab CDS, OpenLab ChemStation, OpenLab EZChrom
      • Data Management Software
        OpenLab ECM, OpenLab ECM XT, OpenLab Server
      • Mass Spectrometry Software
        Data Analysis, Data Acquisition, Productivity App, WalkUp Software
    • Vacuum
  • Applications Areas
    Applications Areas
    • Basic Research
    • Energy and Chemicals
    • Environmental
    • Food and Agriculture
  • Agilent Knowledge Portal
  • Links
    Links
    • Collection of Support Resources
    • Webinar Notifications
    • Contact Support
    • User Contributions
  • Getting Started
    Community Help and Resources
  • Browse
    Browse
    • People
    • Places
    • Your View
    • Your Community Inbox
LC/MS Portal
  • Agilent Knowledge Portal
LC/MS Portal
Articles Installing an Ion Capillary in Triple Quadrupole LC/MS Systems Equipped With a Gate Valve
  • Articles
  • Videos
  • Forum
  • Announcements
  • More
  • Cancel
  • Agilent Knowledge Portal
  • LC/MS Portal Home
  • LC/MS Portal Articles
  • LC/MS Portal Videos
  • More
  • Cancel
  • Created :  10 Nov 2022
  • Modified :  21 Jan 2023
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  LC/MS Systems
  • Component :  LC/MS Triple Quadrupole
  • Product Name :  6475 Triple Quadripole LC/MS 6495 Triple Quadripole LC/MS Ultivo Triple Quadrupole LC/MS
  • Task :  Maintenance
Related Articles
  • Removing the Ion Transfer Capillary from Triple Quadrupole LC/MS Systems Equipped With a Gate Valve

    This Information Applies To: Agilent 6400 Series triple quadrupole LC/MS systems equipped with a gate valve. Issue Removing the Ion Transfer Capillary for maintenance on instruments with gate valve. The gate valve allows ion capillary removal and installation without having to vent the instrument. Required Items Clean powder-free nitrile gloves Capillary puller tool (supplied with your triple quadrupole LC/MS with a gate valve installed) Steps to follow Caution : Handling...
  • What to do if my LC-QQQ Autotune Fails?

    This Information Applies To: Agilent 6400 Series triple quadrupole LC/MS systems Issue Autotune fails to complete. Background Agilent 6400 LC/MS systems use an Autotune algorithm to optimize the sensitivity and mass resolution of the instrument. Sometimes when running the autotune procedure the autotune does not complete and the user is given an Autotune failed message in MassHunter. One of the main reasons for Autotune failure is a lack of sensitivity or abundance of the tuning ions...
  • Removing the Ion Transfer Capillary from Agilent 6000 Series LC/MS Systems

    This Information Applies To: All Agilent 6000 Series LC/MS Systems without a gate valve for ion capillary installation/removal. For instructions for removing an ion capillary on LC/MS instruments with a gate valve installed, please click here Issue: Removing the Ion Transfer Capillary for maintenance. Steps to follow: Tips : The figures in this article show an LC/MS with an Agilent Jet Stream (AJS) source installed. The process of capillary removal is the same for LC/MS instruments...
  • Installing the Ion Transfer Capillary in Agilent 6000 Series LC/MS Systems

    This Information Applies To: All Agilent 6000 Series LC/MS Systems without a gate valve for ion capillary installation/removal. Issue: Installing the Ion Transfer Capillary after cleaning or replacement. For instructions for installing an ion capillary on LC/MS instruments with a gate valve installed, click here Steps to follow: Tips : The following figures show an LC/MS with an Agilent Jet Stream (AJS) source installed. The process of capillary installation is the same for...
  • How To Ping the IP Address of an Agilent GC System

    This Information Applies To: Agilent GC Systems 6890N, 7890A/B, 7820, 8860,8890, Intuvo 9000 Issue The communication between Agilent GC instrument and the computer (PC) requires an active LAN connectivity. Each part of the instrument-PC network has a unique IP Address, a shared Subnet Mask, and an undefined Gate Way. This ping test verifies the LAN connectivity between PC and instrument. If the pinging is not successful, the customer will not be able to configure the new instrument or the configured...

Installing an Ion Capillary in Triple Quadrupole LC/MS Systems Equipped With a Gate Valve

Answer

This Information Applies To: Agilent 6400 Series triple quadrupole LC/MS systems equipped with a gate valve


Issue

Installing the Ion Transfer Capillary after cleaning or replacement in Agilent LC/MS instruments with a gate valve.


Required Items

  • Clean powder-free nitrile gloves
  • LC/MS-grade isopropanol
 Warning 
Caution: Ensure the source heated zones are cool before working on the instrument.

Before installing the new ion capillary, the used ion capillary must be removed from the instrument. See Removing the Ion Transfer Capillary from Triple Quadrupole LC/MS Systems Equipped With a Gate Valve .

Steps to follow

 Warning 
Caution: Handling Ion Source components or the Ion Transfer Capillary without gloves can cause contamination. Please use clean powder-free nitrile gloves during this procedure

1. Put on clean powder-free nitrile gloves.

2. Lubricate the ion capillary entrance end with LC/MS-grade isopropanol. See Figure 1. Check the correct orientation of the capillary before inserting it into the instrument. The black band on resistive capillaries is positioned toward the front (spray chamber side) of the instrument.


Figure 1. Arrow illustrating the correct end of the resistive capillary to apply IPA.

 Warning 
Caution: Putting vertical or horizontal pressure on the capillary can break it.

3. Carefully insert the capillary straight into the desolvation assembly until it stops against the entrance of the gate valve . See Figure 2.


Figure 2. Insert the capillary straight into the desolvation assembly.

4. Slide the gate valve actuator lever lock button towards the back of the instrument with one finger. With the other hand, push down the actuator lever until it fully stops, then release the actuator lever lock button. See Figure 3.


Figure 3. Opening the gate valve using the actuation lever.

5. Fully press the capillary into the desolvation chamber.

 Warning 
Caution: Do not twist or turn the capillary cap when you install it or you can damage the metal plating on the end of the capillary.
In the next step, avoid spraying any IPA directly into the ion capillary entrance. Use a small amount of IPA applied from the side to avoid spraying liquid directly into the capillary.

 

6. Wet the end of the ion capillary with LC/MS-grade isopropanol, then install the capillary cap onto the end of the capillary. Then screw on the spray shield as shown in Figure 4.


Figure 4. Ion transfer Capillary insertion

10. Close the spray chamber and then bring the source back up to your method temperature and gas flow setpoints.

11. Wait a minimum of 2 hours before using the instrument for analysis to let the heated zones in the instruments fully equilibrate.

 Tip 
Learn how to effectively maintain your Agilent LC/MS:
LCMS-6400-1201s - Essential Housekeeping Tasks and Best Practices for Agilent 6400 Series LC/TQ e-learning course available from Agilent education
 
Was this helpful?
  • Yes
  • No
Thank you for your feedback!

Agilent Community Feedback

Agilent Community Feedback

×
We are glad this was helpful! We are sorry this was not helpful. If you still need assistance please create a community post or contact support. To help us improve, please provide any additional feedback. For full details of how we will treat your information, please view our privacy policy.
Submit Cancel
Submit Cancel
Privacy Statement
Terms of Use
Contact Us
Site Help