How to Install a Max-Light Cartridge Cell?

Answer

This Information Applies To: Agilent LC Systems with G4212x / G7117x diode array detectors (DAD)

Issue

The Max Light cartridge flow cells have small internal diameter inlet and outlet capillaries. As a consequence, particles can easily block the capillary with some application/ practices, or using a dirty column, dirty solvents, or by flushing the system through the flow cell.

If it is leaking or if the intensity drops due to a contaminated flow cell, the cartridge needs to be replaced.

Items Required

• Wrench, 1/4 inch for capillary connections or
• 5043-0915 Fitting mounting tool for bio-inert capillaries

Steps to Follow

1. Recover the front cover.
2. Locate the cell area
3. Disconnect the inlet capillary to CELL-IN (left) and the waste tubing to CELL-OUT (right).
4. Unlock the cell cartridge holder by pulling the lever to the front.
5. The lever should be in the final down position.
6. Pull the cell cartridge holder completely out towards the front.
7. Remove the cell from the cartridge holder.
8. Replace the black hoods [A] to the cell interfaces (in/out) and insert plugs [B] for save storage.
9. Remove the black hoods from the cell interfaces (in/out) and insert the cell into the cell cartridge holder.
10. Slide the cell cartridge holder completely into the module.
11. Lift the two levers into the upper final position to fix the cell.
12. Remove the plugs from the CELL-IN and CELL-OUT (keep them safe).
13. Connect the inlet capillary to CELL-IN (left) and the waste tubing to CELL-OUT (right).
    

    Tips: Perform a Cell Test and a Wavelength Verification Test before launching any analyses. It is performed before step 14 in case the cell's installation has to be adjusted.

14.  Close the front cover.

Learn how to effectively your Agilent LC system: HPLC-1290-2250z - Replacing the Cartridge Cell in an Agilent 1290 G4212X Diode Array Detector (DAD) e-learning course available from Agilent education.