How to Remove and Reinstall the Loop Capillary in G4226A/G1367E High Performance (HiP) Autosamplers

Answer

This Information Applies To: Agilent 1290 Infinity (G4226A) and Agilent 1260 Infinity (G1367E) HiP Autosampler

Issue

Replacing the Loop Capillary of the Autosampler is required when it is broken or leaking.

Items Required

• Wrench open 1/4 - 5/16 inch (p/n 8710-0510)

For an Agilent 1290 Infinity (G4226A) HiP Autosampler, one of the following:

• Loop cartridge 20 µL, Red Capillary (p/n G4226-60310)
• Loop cartridge 40 µL, Green Capillary (p/n 5067-4703)
• Loop cartridge 100 µL, Blue Capillary (p/n 5067-4710)

For an Agilent 1260 Infinity (G1367E) HiP Autosampler, one of the following:

• Loop cartridge 40 µL, Green Capillary (p/n 5067-4703)
• Loop cartridge 100 µL, Blue Capillary (p/n 5067-4710)

Steps to Follow 

Warning: This procedure risks exposure to hazardous, toxic, or flammable solvents and reagents. When working with these substances observe safety procedures as described in the material handling and safety data sheet supplied by the chemical vendor (for example by wearing personal protective equipment). Always follow good laboratory practice.

Figure 1 shows main assemblies of an Agilent G4226X/G1367E High Performance (HiP) Autosamplers

Figure 1. Main Assemblies of an Agilent G4226A/G1367E High Performance Autosamplers.
1. Needle Assembly, 2. Needle Carrier, 3. Transport Assembly, 4. Blocked needle seat, 5. Loop capillary, 6. Analytical Head, 7. Leak parts, 8. Needle seat

1. Finish any pending or running acquisition job.

2. Stop the flow at the pump and remove the solvent lines from the eluent bottles to avoid spilling solvent.

3. Close the shutoff valves at the pump if available on your pump module.

4. In the Agilent Lab Advisor Software, connect to your LC system then click Service and Diagnostics> Autosampler> Maintenance Positions> Change Loop capillary. Instructions on downloading and installing Lab Advisor on your Workstation PC are provided in the article How to Install Lab Advisor.

5. Click Start to start the maintenance task. Confirm the part number for the loop capillaries listed in the pop-up configuration window in the Lab Advisor maintenance task match the new loop capillary being installed in your Autosampler. (Figure 2)

   
   
   Figure 2. Running the Change Loop Capillary maintenance task in Lab Advisor. 
   
   

6. Open the Autosampler front door and remove the leak tubing.

7. Unscrew the loop capillary from the top of the analytical head then open the loop holder cover and unwind the loop capillary from loop holder. Release loop Tag from Loop holder. (Figure 3)

   
   
   Figure 3. Unscrewing and removing the Loop Capillary from the Loop Holder.
   
   

8. Open the Leak Guide then remove the Loop Cap and disconnect the loop from the needle. (Figure 4)  

   
   
   Figure 4. Disconnecting the loop from the needle.
   
   

9. Push the Loop cartridge downwards through the transport arm and slide it out together with the loop capillary (Figure 5)  

   
   
   Figure 5. Removing the Loop Capillary from the Autosampler Transport Arm.
   
   

10. Guide the loop capillary through the Transport Arm and push the loop cartridge into place and insert the loop Cap (Figure 6)  

    
    
    Figure 6. Guiding the loop capillary through the Transport Arm.
    

11. Reconnect the loop to the needle and close the leak guide.
12. Attach the Loop tag to the Loop holder (Figure 7) and Wind the loop capillary into the loop holder clockwise (Figure 8)

    
    
    Figure 7. Loop tag on the Loop holder
    
    

    
    
    Figure 8. The position of the loop holder 
    
    

13. Reconnect the loop capillary to the fitting at the top of the metering device and close the 1290 Autosampler front door.
14. Go back to Lab Advisor and click End to finish the Maintenance Task. Click System Overview and then click Disconnect to disconnect your instrument from Lab Advisor. (Figure 9)

    
    
    Figure 9. Closing the Change Loop Capillary maintenance task and disconnecting the instrument from the Lab Advisor.
    
    

    Tips: If possible, run a system pressure test with the port 6 of the injection valve blanked with the appropriate blanking nut. This test will ensure that the autosampler flow path is pressure tight to a pressure higher than your application requires.

     

    Learn more how to effectively replace the Loop Capillary on an Agilent G4226A/G1367E Agilent HiP Autosampler:  HPLC-Replacing the Loop Capillary on an Agilent G4226X/G1367E Agilent HiP Autosampler e-learning course available from Agilent education.