• Created : 
  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Automated Electrophoresis Software
  • Component :  Femto Pulse Analysis Kits and Reagents
  • Product Name :  BAC Kits Femto Pulse instrument controller software ProSize data analysis software
  • Task :  Data Acquisition Data Analysis Troubleshooting
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BAC Method Missing from the Agilent Femto Pulse Software

Answer

This Information Applies To: Agilent Femto Pulse, Agilent Femto Pulse controller software 1.0, ProSize analysis software 3.0, Agilent Genomic 55 kb BAC kit (p/n FP-1003-0275)

Issue:

In Agilent Femto Pulse controller software version 1.0, it is not possible to select the FP-1003-22 55 kb BAC method from the Separation Set up dialog box. This situation occurs due to the BAC method and configuration files not being included in the Femto Pulse controller version 1.0 and ProSize 3.0 software installers.

Steps to follow: 

Download the latest Femto Pulse controller and ProSize software here.

Alternatively, if you do not wish to upgrade to the latest software versions it is possible to download the 55 BAC method and configuration files.

Download the method file here and the configuration file here.

  1. Copy the method file (.mthds) into the folder C:\AATI\Methods\22cm.
  2. Copy the configuration file (.ini) into the Configurations folder within your ProSize 3.0 directory, e.g. C:\ProSize 3.0\Configurations\FP.

Once the 55 Kb BAC.mthds and .ini file are copied into the correct folders, and the software is restarted, the BAC method will be available in the software.

To run the BAC method, click Add to queue in the Experimental Run Controls. The method can be run for the selected group or the entire tray (see Figure 1).



Figure 1. Experimental Run Controls

Clicking Add to queue opens the Separation Set up dialog box. In the Method drop-down list, select FP-1003-22 - 55 Kb BAC.mthds (see Figure 2).



Figure 2. Separation Set up dialog box

 Tip 
Learn how to effectively operate your Agilent Femto Pulse:

For Research Use Only. Not for use in diagnostic procedures.

PR7000-8125

 
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