Peak Width Adjust error: Gain is at minimum and the abundance is too large!

Hi Daniel,

I hope you are well. From what I have you probably have the wrong horn. The white one is the Low Gain EM replacement horn, LCMS G1960-80103 I believe was meant for the later versions of the 6100s Whilst the green one which should be the right one for this system is the High gain version Electron multiplier replacement horn PN: 05971-80103. I haven't worked on this product for a while but I know from past experience that crazy things happen when the wrong horn is used even if they look somewhat compatible. I suggest you get in contact with your FSE maybe they can seek clarification from FSG on your behalf. I however think you have the wrong horn. Good luck.

Thank you for your answer. I just emailed Agilent and copy-pasted your point.

For now, I put the old EM horn, and still working. I will wait to get an answer from Agilent before trying again.

Thank you so much for the warning!! I will post the outcome.

I can confirm this is the correct horn for a 6100 SQ.

It may just be too good as it is so new especially if the old EMV setting is still applied

As Kevino said you can see this with the wrong horn too (The high gain one used in QQQ)

Try the following to reduce the EMV applied to the horn.

1. Set the Gain Curve at 1000V by entering MSD Tune View
2. Select the Mode.
3. Type SetGain 1000 in the ChemStation command line and hit Enter key,.
4. Begin Autotune.
5. If needed verify with Checktune after successful autotune.

If the above does not work, the EM horn can be "burned in" by performing a Single Ion Profile overnight with EM set at 2500V, LC mobile phase directly going into nebulizer.

Thanks Ross. This is very helpful. @Daniel please let us know if this helps. Thanks.

Thank you for your help. This is very helpful. I will try as soon I have access to the instrument and let you know.

I will! Thanks!

Ok, I followed the instructions, set the voltage 1000V and autotune. I got a nice tune result. Injected a real sample, and it was good.

Solved. Thank you!