Tuning no signal

That's more an MS question, so you might want to post your issue in a different forum.

Beside from that, is your MS a Single Quad? G6100 series? Do you get any error message from the software, something like: "Difficulty with spray chamber electronics"? If your spray chamber settings are okay, you have verified that a sufficient amount tuning mix makes it to the nebulizer and you're using a fresh bottle of the correct mix, then I would also make that the corona needle is clean and sharp, the nebulizer is adjusted correctly and the spray shield is clean.

Thanks for replying. I will post this too on MS forum.

Yes. single quad, and sorry forgot the details. The instrument is Agilent LC-MS (1260 HPLC and 6120 MSD). Other details,

I mostly get error message of "Peak width adjust error: the valid range for width gain is -2047 to 2047" and the chromatogram shows bunch of small peaks. 

Thanks for replying. I will post this too on MS forum.

Yes. single quad, and sorry forgot the details. The instrument is Agilent LC-MS (1260 HPLC and 6120 MSD). Other details,

I mostly get error message of "Peak width adjust error: the valid range for width gain is -2047 to 2047" and the chromatogram shows bunch of small peaks. 

Thanks for sharing the details. Yes, your case would require more of an MS person; hope you will get some advice on the MS forum. Personally I would check the spray chamber parameters and the reported values, but also take a look at the spray shield.