I am having trouble in stabilizing my RID detector. Please provide me proper procedure for stabilization and getting good baseline in RID. Also when i am joining the column or removing the column there is no effect on the RID baseline.
Find maintenance, best practice, and troubleshooting articles.
I am having trouble in stabilizing my RID detector. Please provide me proper procedure for stabilization and getting good baseline in RID. Also when i am joining the column or removing the column there is no effect on the RID baseline.
All of the previous advice is very good. I do all of these things, too.
In addition:
1. I place a box over the HPLC solvent bottles to provide a buffer against temperature fluctuations and air currents (HVAC off-on cycles). The boxes that vendors ship 4x4-L solvent bottles works great.
2. At another lab, I had to fashion a cardboard baffle, packing taped to ceiling vents to direct air currents away from blowing directly on the HPLC system / RID.
3. I tightly swath all exposed flow path lines in small plastic bubble wrap. This includes the waste line, as it can amazingly radiate temperature changes back to the detector.
4. A vendor taught me how to coil metal HPLC line tightly around a pencil, attach it to column, then fit it inside the heater with the column (a pre-conditioner). It took a couple of tries to determine the best i.d. and length.
I know you are expecting me to say that I stand on my left foot while chanting during the RID analysis. . . Tempting at times, but not yet. These are the things that I had to add over a few years of working with RID analyses, older building / HVAC / electrical, etc.
I hope this helps. Good luck with your RID.
Update: I place a box over the solvent bottles (only) that are located in a tray on top of the LC stack. The box is not placed over any of the individual LC units in the stack, since this may cause overheating. This is a similar concept to what graettin suggested with the bigger solvent reservoir to buffer against temperature changes.
Update: I place a box over the solvent bottles (only) that are located in a tray on top of the LC stack. The box is not placed over any of the individual LC units in the stack, since this may cause overheating. This is a similar concept to what graettin suggested with the bigger solvent reservoir to buffer against temperature changes.