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Same sample injections with huge difference in response, what could cause this?

raybrew

Hello, 

we currently are using1290 Infinity II LC and we are having a problem with injections with in the same sample but the response being significantly different between the two. We have changed the needle, cleaned the source and checktune the instrument and are currently recalibrating to see if that fixed the problem. If not do you have any other suggestions on what we can do, we just do not trust the results. Thanks for you help.

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    Yes the retention time is okay and the peaks and baseline look okay. The needle and needle seat were just changed on our last PM (February 14th). The autosampler model number is G7167B. I will have to check on the rotor seals or metering seal.

    Our calibration comes in fine, the response is where it should be and we can get maybe one or two samples ran with not hiccup. Then we will get the fluctuation in the response. For our samples we test a sample, duplicate sample, spike sample and blank all with internal standard. The sample response would be ~850 where the duplicate sample response would be ~150 and the sample is prepared the exact same way.  

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  • 0

    Yes the retention time is okay and the peaks and baseline look okay. The needle and needle seat were just changed on our last PM (February 14th). The autosampler model number is G7167B. I will have to check on the rotor seals or metering seal.

    Our calibration comes in fine, the response is where it should be and we can get maybe one or two samples ran with not hiccup. Then we will get the fluctuation in the response. For our samples we test a sample, duplicate sample, spike sample and blank all with internal standard. The sample response would be ~850 where the duplicate sample response would be ~150 and the sample is prepared the exact same way.  

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