Same sample injections with huge difference in response, what could cause this?

Question

Hello, 
 we currently are using1290 Infinity II LC and we are having a problem with injections with in the same sample but the response being significantly different between the two. We have changed the needle, cleaned the source and checktune the instrument and are currently recalibrating to see if that fixed the problem. If not do you have any other suggestions on what we can do, we just do not trust the results. Thanks for you help.

fgunawan · Accepted Answer

Thank you raybrew for your reply. In this case, it would be best to continue with the help from your local Agilent Technician. Since a service visit has been started, we will close this thread and allow the field support team to assist you with the rest.

SAGAR8848 · Answer

There may be two reason behind it 1) there may be carryover of sample from previous so set needle wash after every injection 
 2) check the analytical method , sometime the unvalidated method also shows this of problem 
 another suggestion : decrease the sample injection volume may be it can solve. it also happen once in my lab too and above method solve and onwards no problems seen yet

Same sample injections with huge difference in response, what could cause this?

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