Hi dear
I have a USP standard of a compound and i tested it with HPLC (uv : 260 nm)
I read the concentration of API about 121%
would you please guiding me why it was happened?
Find maintenance, best practice, and troubleshooting articles.
Hi dear
I have a USP standard of a compound and i tested it with HPLC (uv : 260 nm)
I read the concentration of API about 121%
would you please guiding me why it was happened?
Hi
Purge complete system( remove column and connect the line with union before purging )...
Hi,
ISH is correct, purging your system will remove any residual compound that may be stuck to the column or flow cell. After flushing, if you still observe very high values then it could be that your sample concentrations are not as indicated or that your method defines an injection volume that is different from reality. You can try weighing the vial before and after injection to verify if the autosampler is taking consistent aliquots.
Are you using the USP standard as your calibration standard and measuring an equivalent concentration of your API at the same nominal concentration and seeing the result to be 121% of what it should be?
Is the reference standard and your API in the same salt forms? Differences like this can be seen when using measuring free-bases against salts.
Andy