HPLC Baseline Drifting down at start of each injection


Please forgive me as I am still new to this but I have not found the answer to my question.

After injection, the baseline drifts downward but it still shows the peaks. The mobile phase is fresh, the column is less than 6 months old. I just replaced the Deuterium lamp. Is there somewhere else I should start looking to hopefully resolve this issue? Please let me know if you need any more details to possibly help me out.

Thank you very much for any help, I couldn't do what I do without you all


  • Hi

    After replacing d2 lamp, you should do wavelength calibration..

  • Check above detail info to see other options.

  • Is there any noise coming out from system?

Reply Children
  • Hello,

    I apologize for the delay in responding, you know how lab life is. I tried to look around to see how to calibrate the wavelength but was unable to find it with a quick search. Our Lab Advisor software is not working and my boss does not want to update for now. Hopefully there is still a way to do that.

    Also, yes there is some noise. I can try to get you a picture some time but am not able to at the moment.

    I appreciate all of your help.


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