• State Verified Answer
  • +1 person also asked this people also asked this
  • Replies 10 replies
  • Subscribers 94 subscribers
  • Views 4537 views
  • Users 0 members are here
Related

Agilent Knowledge Portal

Find maintenance, best practice, and troubleshooting articles.

Agilent Knowledge Portal

HPLC Baseline Drifting down at start of each injection

orangiraff24

Hello

Please forgive me as I am still new to this but I have not found the answer to my question.

After injection, the baseline drifts downward but it still shows the peaks. The mobile phase is fresh, the column is less than 6 months old. I just replaced the Deuterium lamp. Is there somewhere else I should start looking to hopefully resolve this issue? Please let me know if you need any more details to possibly help me out.

Thank you very much for any help, I couldn't do what I do without you all

KH

Parents
  • 0

    Hi

    After replacing d2 lamp, you should do wavelength calibration..

  • +1 in reply to ISH

  • 0 in reply to ISH

    Check above detail info to see other options.

  • 0 in reply to ISH

    Is there any noise coming out from system?

Reply Children
  • 0 in reply to ISH

    Hello,

    I apologize for the delay in responding, you know how lab life is. I tried to look around to see how to calibrate the wavelength but was unable to find it with a quick search. Our Lab Advisor software is not working and my boss does not want to update for now. Hopefully there is still a way to do that.

    Also, yes there is some noise. I can try to get you a picture some time but am not able to at the moment.

    I appreciate all of your help.

    KH

Was this helpful?
Privacy Statement
Terms of Use
Contact Us
Site Help