HPLC Baseline Drifting down at start of each injection


Please forgive me as I am still new to this but I have not found the answer to my question.

After injection, the baseline drifts downward but it still shows the peaks. The mobile phase is fresh, the column is less than 6 months old. I just replaced the Deuterium lamp. Is there somewhere else I should start looking to hopefully resolve this issue? Please let me know if you need any more details to possibly help me out.

Thank you very much for any help, I couldn't do what I do without you all


  • Hi

    After replacing d2 lamp, you should do wavelength calibration..

  • Check above detail info to see other options.

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