LCMS column pressure

Hi

What mobile phase you are using.

When you are purging the system by opening purge valve of pump that time pressure stable or not.If pressure stable then close this purge valve.

After that autosampler valve to put in bypass mode from masshunter chemstation then see pressure stable or not.

And last remove column and connect capillary tubing with union to see pressure stable or not to identify column is clogged or not.

Hi

What mobile phase you are using.

When you are purging the system by opening purge valve of pump that time pressure stable or not.If pressure stable then close this purge valve.

After that autosampler valve to put in bypass mode from masshunter chemstation then see pressure stable or not.

And last remove column and connect capillary tubing with union to see pressure stable or not to identify column is clogged or not.

Basically, we have to identify which module is giving problem.

Even line filter in mobile phase bottle also may clogged.

You can check flow by using any measurinģ vial and stop watch to see flow is okay or not..

https://community.agilent.com/technical/lc/w/wiki/7687/troubleshooting-high-pressure-issues-in-agilent-lc-systems

For more detail, you can check above steps.

thank you for your reply.

I am using mobile phase A 20mM NH4OAc/Milli Q water and 100% Methanol mobile phase B.

when I upload the method with which I am supposed to run my samples. In the start, the pressure goes up and then stabilizes, when all the parameters become ready.

Means pressure fluctuates..

Would it be possible to regenerate the column if the problem persist?

Without column, it's stable or not.

If without column, it's stable then column has some problem.