LCMS column pressure

Hi

What mobile phase you are using.

When you are purging the system by opening purge valve of pump that time pressure stable or not.If pressure stable then close this purge valve.

After that autosampler valve to put in bypass mode from masshunter chemstation then see pressure stable or not.

And last remove column and connect capillary tubing with union to see pressure stable or not to identify column is clogged or not.

thank you for your reply.

I am using mobile phase A 20mM NH4OAc/Milli Q water and 100% Methanol mobile phase B.

when I upload the method with which I am supposed to run my samples. In the start, the pressure goes up and then stabilizes, when all the parameters become ready.

thank you for your reply.

I am using mobile phase A 20mM NH4OAc/Milli Q water and 100% Methanol mobile phase B.

when I upload the method with which I am supposed to run my samples. In the start, the pressure goes up and then stabilizes, when all the parameters become ready.

Means pressure fluctuates..

Would it be possible to regenerate the column if the problem persist?

Without column, it's stable or not.

If without column, it's stable then column has some problem.

And pressure fluctuates then chances that pump has issue.

May be frit of purge valve. Or pump head.

You can condition the column after passing small amount of mobile phase for 4 to 10 hour..

https://community.agilent.com/technical/lc/w/wiki/7775/general-column-regeneration-procedure

You can follow above procedure to regenerate the column if problem persist with column.