Hplc

Hi,

It could be normal for your method. Have you tried to do a calibration and see if the response is linear?

Yes I have a linear response.

Thank you.

An excellent and quick way to check linearity of an RID is the Refractive Index Detector OQ/PV Test Sample (5064-8220). It consists of

• 5 mg/mL
• 10 mg/mL
• 15 mg/mL
• 25 mg/mL
• 50 mg/mL

Simply use a 2m SST restriction capillary, a flow of 1ml/min (water) and a run time of 1 min. Injection volume would be 2ul.

Thank you