Hplc

Hi, when I inject 1000 ppm( 100%) of sacrose ,I get  this area 57367.

Is it normal to have only 5 number of area? Or I should have more?

I use RID detector.

Thank you

Parents
  • An excellent and quick way to check linearity of an RID is the Refractive Index Detector OQ/PV Test Sample (5064-8220). It consists of

    • 5 mg/mL
    • 10 mg/mL
    • 15 mg/mL
    • 25 mg/mL
    • 50 mg/mL

    Simply use a 2m SST restriction capillary, a flow of 1ml/min (water) and a run time of 1 min. Injection volume would be 2ul.

Reply
  • An excellent and quick way to check linearity of an RID is the Refractive Index Detector OQ/PV Test Sample (5064-8220). It consists of

    • 5 mg/mL
    • 10 mg/mL
    • 15 mg/mL
    • 25 mg/mL
    • 50 mg/mL

    Simply use a 2m SST restriction capillary, a flow of 1ml/min (water) and a run time of 1 min. Injection volume would be 2ul.

Children
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