Hplc

Question

Hi, when I inject 1000 ppm( 100%) of sacrose ,I get this area 57367. 
 Is it normal to have only 5 number of area? Or I should have more? 
 I use RID detector. 
 Thank you

sschombu · Accepted Answer

An excellent and quick way to check linearity of an RID is the Refractive Index Detector OQ/PV Test Sample (5064-8220). It consists of 
 &bull; 5 mg/mL &bull; 10 mg/mL &bull; 15 mg/mL &bull; 25 mg/mL &bull; 50 mg/mL 
 Simply use a 2m SST restriction capillary, a flow of 1ml/min (water) and a run time of 1 min. Injection volume would be 2ul.

smailchimiste83 · Answer

Yes I have a linear response. 
 Thank you.

smailchimiste83 · Answer

Thank you

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