What is the basic maintanance that i can do to ensure the HPLC working well?

Agilent used to have a product called Agilent Maintenance Wizard which guides you through the preventive maintenance procedure, however we stopped selling it ...

Things to look at:

Pump: Leak (use Leak Test in Lab Advisor) and Ripple, if there is a leak, ensure everything is tight and if the leak occurs on the seals, exchange them! Ripple can point at problems with the valves in the pump which can be exchanged as well, but mostly this is caused by air bubbles or wrong compression settings.
Run a elasticity calibration if available if you changed parts on the pump drive or disassembled it.
Injector: The rotor in the valve of the injector as well as the seat and needle can be exchanged as they all wear, there is a Sampler Leak test for most samplers which can give an indication.

Column Compartment: Well either it works or it doesn´t, there is a test to test the performance of the heating / cooling elements, you can run it, but normally it doesn´t matter as long as you can reach the temperature required for your application
Detector: Depending on detector the UV-Lamp has to be exchanged if you no longer reach the Signal to Noise level you require for your application! There is buildup of deposits on the optical windows of the detectors, cleaning these windows or replacing them helps with sensivity and might contribute to a large signal / noise increase.

For some detectors you can run a wavelength calibration inside Lab Advisor.

Lab Advisor is on the CD delivered with your Pump, there is no additional license required for basic maintenance of up to 10 modules.

In case you don´t have the cd / don´t have a CD-Drive you can download the latest version of Lab Advisor here:  http://labadvisor.pl29.agilent.com/latest

B.t.w. part wear greatly depends on the pressure you use the system at as well as the solvents you use..

Hi Shahrul,

What LC modules do you have?  What version of software?

We have videos here in the Agilent Community and some older maintenance videos for the 1100/1200 are here:  http://www.chem.agilent.com/cag/cabu/lcvideoindex.asp

Here is an older but good document Tips and Tricks of LC user maintenance:

https://www.agilent.com/cs/library/usermanuals/public/Tips%20and%20Tricks%20of%20LC%20User%20Maintenance_021910.pdf

There are user manuals on the Agilent.com website.... if you search for the model and "user".

There are tests that you can run from Lab Advisor  http://labadvisor.pl29.agilent.com/latest   a handheld controller or old Chemstation.

The latest version of lab advisor does not work on older operating systems..

The main things I look at are:

Pump:  is the pressure stable and "normal, how is the pressure ripple? Does it pass the Leak or Pressure test?  Are the retention times of the standards reproducible?

Autosampler:  Pressure test pass?  Are the areas of the standards reproducible?

Column Compartment: Run the Thermostat test.

Detectors: run the built in tests

my HPLC is agilent 1100 series DAD.. thanks for your recommendation

Thank for your help.. really appreciate it.

You can read about the maintenance of the DAD in the usermanual as well get it here: https://www.agilent.com/cs/library/usermanuals/public/G1315-90006_DAD-A_B_User_ebook.pdf

starting on page 100 the maintenance is described.. Especially when disassembling the flow cell it´s hard to get it right on the first try if you have like 10 tiny parts on the table.. The tiny washers are acutally plate springs and have to be in the correct orientation to work, when pulling them out use a toothpick so they stay in the correct order.

For the pump make sure the PTFE frit in the purge valve gets replaced regularly-about every 2 months or more often if needed.