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Error in autotune! Adjust Peak Width Failed

TheoJooste

Good day Agilent community. We encountered an error in autotuning our GC-QQQ 7890B, in which the following error was stated: "Error in autotune! Adjust Peak Width Failed". My supervisor has tried numerous of other parameters such as doing a manual autotune and we found out the problem arises at MS1, due to check tune indicating that the low, mid and high Isotope mass ratio was a bit outside the parameters. What could possibly be the problem with MS1 and is there any way to fix this before contacting a technician for a full service. Thank you all kindly.

*Edit* The peaks look like baseline/noise and are not resolved during autotuning. My supervisor was able to get our 69 peak resolved during manual tuning and playing around with the quad1 DC voltages however, the peaks went back noise/baseline once we tried autotuning again.

  • 0

    Please share the autotune report. This is the checktune which is not as helpful for troubleshooting.  

  • 0 in reply to paulsalverda

    Hi Paul, thank you for your reply. I can't attach a full autotune report due to it failing mid run. I can however add the text file it generated.

    Fullscreen Autotune_20230419_130648.txt Download 
    --------------------------------------------------------------------------------
Autotune - Starting - 4/19/2023 1:07:20 PM
      Software Revision MassHunter GC/MS Acquisition B.07.05.2479
      PC Hostname DESKTOP-QRSMVU9
      GCQQQ 7010B US1718W001 DSP: 7000.3509, qqqServer: G.7000.058-RUN
      Initial tune file D:\MassHunter\GCMS\1\7010\atunes.eihs.tune.xml

--------------------------------------------------------------------------------
Tune parameters
  MS1DC 6.4
  MS1PostFilterDC 6.4
  MS1 Mass Axis Offset Dynamic
    enabled True
    69, -1.774, 219, -1.737, 264, -1.762, 414, -1.786, 502, -1.779, 
  MS1MassGain -0.42
  MS1 Width Offset Dynamic
    enabled True
    69, -0.015, 219, -0.002, 264, -0.005, 414, -0.008, 502, -0.013, 
  MS1AMUGain 20.9
  MS1Heater 150
  MS2DC -4.6
  MS2PreFilterDC -14.6
  MS2 Mass Axis Offset Dynamic
    enabled True
    69, -1.882, 219, -1.798, 264, -1.836, 414, -1.851, 502, -1.883, 
  MS2MassGain 10.61
  MS2 Width Offset Dynamic
    enabled True
    69, -0.016, 219, -0.006, 264, -0.005, 414, -0.006, 502, -0.011, 
  MS2AMUGain 13.7
  MS2Heater 150
  CCEntranceDC 7.4
  CCHexapoleDC 6.4
  CCHexapoleRFPeak 400
  CCHexapoleDeltaV -5.0
  CCExitDC 0.4
  CCGasOn 1
  Iris Dynamic
    enabled True
    69, -3.5, 219, -12, 264, -16.5, 414, -26, 502, -30.5, 1050, -58.5, 
  HED -10.0
  EMV 1478
  CollisionEnergy 0
  SourceHeater 230
  Repeller 16.9
  IonBody 19.8
  IonFocus -220.0
  Entrance Lens Dynamic
    enabled True
    69, -15.59995, 264, -16.99995, 414, -19.79996, 502, -21.99997, 
  Filament 2
  Emission 100.0
  ElecEnergy 70
  Extractor Dynamic
    enabled True
    69, 14.60002, 264, 14.90002, 502, 14.60002, 
  SensitivityMode 1
  EinzelLens1 0.0
  EinzelLens2 -12.0
  GasSelect B
  GasFlow 0
  FastScanOffset -4.0
  H2Flow 0.00
--------------------------------------------------------------------------------

--------------------------------------------------------------------------------
Acquisition Settings
    MS1ScanMode = TTI
    MS1TTICutoff = 75
    MS1SIMIon = 264
    MS1ScanLowMass = 35
    MS1ScanHighMass = 650
    MS1ScanStep = 0.1
    MS1ProfileMasses = 69.00, 10.00
    MS1ProfileMasses = 264.00, 10.00
    MS1ProfileMasses = 502.00, 10.00
    MS2ScanMode = Profile
    MS2TTICutoff = 50
    MS2SIMIon = 264
    MS2ScanLowMass = 35
    MS2ScanHighMass = 650
    MS2ScanStep = 0.1
    MS2ProfileMasses = 69.00, 10.00
    MS2ProfileMasses = 264.00, 10.00
    MS2ProfileMasses = 502.00, 10.00
    ProfileSamples = 256
    ProfileAverage = 1
    ProfileStep = 0.1
    ScanSamples = 16
    ScanAverage = 3
    ScanThreshold = 0
    ScanIsCentroided = True
    SIMDwellTime = 20
    CaptureSamples = 32000
    CaptureDwellTime = 0.016
--------------------------------------------------------------------------------
      Doing EI HES autotune
      Repeller set to 12
      IonBody set to 10
      IonFocus set to -180
      Extractor set to 12
      EntLens set to -20
      MS1PostFilterDC set to 5
      Iris set to -35
      Lens 1 Offset set to -10
      Lens 2 set to -25
      Filament Select 2

--------------------------------------------------------------------------------
Find Peaks, Quad 1 - Starting - 4/19/2023 1:07:20 PM
--------------------------------------------------------------------------------
      Find Peaks mass 68.08, abundance 121.2, peak width 7.29

--------------------------------------------------------------------------------
Check Abundance, Quad 1 - Starting - 4/19/2023 1:07:29 PM
      Mass 69.00, Limits 100000 to 2000000
             EMV,      mass, abundance, peak width
            1478,     66.86,        118,      9.86
            1678,     72.01,        589,      8.65
            1878,     69.13,       2728,      4.88
            2078,     69.19,       9380,      9.62
            2278,     68.98,      25904,      4.94
            2478,     69.12,      71019,     11.93
            2678,     72.31,     180491,      9.30
Check Abundance, Quad 1 - Finished
      Find Peaks mass 69.18, abundance 194208.2, peak width 8.52
      Find Peaks mass 498.49, abundance 143799.7, peak width 10.79
      Find Peaks mass 70.29, abundance 173434.9, peak width 8.86
      Find Peaks mass 497.63, abundance 171518.2, peak width 50.83

--------------------------------------------------------------------------------
Check Abundance, Quad 1 - Starting - 4/19/2023 1:07:45 PM
      Mass 69.00, Limits 2000000 to 6000000
             EMV,      mass, abundance, peak width
            2678,     69.09,     169955,     10.80
            2878,     69.00,     426941,    -16.58
            3000,     69.17,     759604,      5.63
            3000,     67.92,     614101,      1.69
            3000,     69.04,     625889,      9.87
            3000,     69.07,     748013,      7.78
            3000,     68.89,     711960,      5.64
            3000,     69.15,     601906,     10.55
            3000,     68.98,     706757,      9.19
            3000,     69.00,     729832,      9.49
            3000,     69.19,     686079,     13.95
            3000,     72.14,     604682,     11.96
            3000,     72.70,     645750,     12.73
            3000,     68.84,     689163,      8.97
            3000,     68.97,     681005,      9.99
Check Abundance failed
Check Abundance, Quad 1 - Finished

--------------------------------------------------------------------------------
Adjust Mass Axis, Quad 1 - Starting - 4/19/2023 1:08:09 PM
      target low mass 69.00, target high mass 502.00, tolerance 1.00, adjustAB False
      axis offset, axis gain,    axis A,    axis B,  low mass, abundance, peakwidth, high mass, abundance, peakwidth
            0.000,      0.00,      1.00,   0.00048,     70.88,  709723.3,     10.66,    490.14,  672599.1,     11.51
Adjust Mass Axis, Quad 1 - failed

--------------------------------------------------------------------------------
Adjust Peak Width, Quad 1 - Starting - 4/19/2023 1:08:13 PM
      target peak width 6.00, tolerance 2.00, adjustAB False
      axis offset, axis gain, pw offset,   pw gain,      pw A,      pw B,  low mass, abundance, peakwidth, high mass, abundance, peakwidth
           -2.000,     25.00,   -0.0127,     20.87,     -2.40,   0.00060,     69.54,    707630,    10.756,    520.63,    633053,    49.137
Adjust Peak Width, Quad 1 - failed

--------------------------------------------------------------------------------
Adjust Mass Axis, Quad 1 - Starting - 4/19/2023 1:08:15 PM
      target low mass 69.00, target high mass 502.00, tolerance 1.00, adjustAB False
      axis offset, axis gain,    axis A,    axis B,  low mass, abundance, peakwidth, high mass, abundance, peakwidth
            0.000,      0.00,      1.00,   0.00048,     70.89,  773630.3,     20.76,    490.31,  635417.2,     49.89
Adjust Mass Axis, Quad 1 - failed

--------------------------------------------------------------------------------
Adjust Peak Width, Quad 1 - Starting - 4/19/2023 1:08:17 PM
      target peak width 6.00, tolerance 2.00, adjustAB False
      axis offset, axis gain, pw offset,   pw gain,      pw A,      pw B,  low mass, abundance, peakwidth, high mass, abundance, peakwidth
           -2.000,     25.00,   -0.5797,     -4.13,     -2.40,   0.00060,     69.60,    769411,    10.045,    499.80,    689953,    51.023
Adjust Peak Width, Quad 1 - failed

--------------------------------------------------------------------------------
Adjust Mass Axis, Quad 1 - Starting - 4/19/2023 1:08:19 PM
      target low mass 69.00, target high mass 502.00, tolerance 1.00, adjustAB False
      axis offset, axis gain,    axis A,    axis B,  low mass, abundance, peakwidth, high mass, abundance, peakwidth
           -2.600,     31.71,      1.00,   0.00048,     68.95,  916784.3,      4.22,    499.22,  629934.3,     33.04
Adjust Mass Axis, Quad 1 - failed

--------------------------------------------------------------------------------
Adjust Peak Width, Quad 1 - Starting - 4/19/2023 1:08:22 PM
      target peak width 6.00, tolerance 2.00, adjustAB False
      axis offset, axis gain, pw offset,   pw gain,      pw A,      pw B,  low mass, abundance, peakwidth, high mass, abundance, peakwidth
           -2.600,     44.88,   -1.3000,    -29.13,     -2.40,   0.00060,     69.88,    935183,     3.111,    515.38,    656675,     9.729
Adjust Peak Width, Quad 1 - failed

--------------------------------------------------------------------------------
Adjust Mass Axis, Quad 1 - Starting - 4/19/2023 1:08:24 PM
      target low mass 69.00, target high mass 502.00, tolerance 1.00, adjustAB False
      axis offset, axis gain,    axis A,    axis B,  low mass, abundance, peakwidth, high mass, abundance, peakwidth
           -1.492,     19.88,      1.00,   0.00048,     70.21,  659646.6,      8.76,    511.01,  684082.9,     41.76
Adjust Mass Axis, Quad 1 - failed

--------------------------------------------------------------------------------
Adjust Peak Width, Quad 1 - Starting - 4/19/2023 1:08:26 PM
      target peak width 6.00, tolerance 2.00, adjustAB False
      axis offset, axis gain, pw offset,   pw gain,      pw A,      pw B,  low mass, abundance, peakwidth, high mass, abundance, peakwidth
           -1.465,     -5.12,   -1.3000,    -54.13,     -2.40,   0.00060,     72.97,    643000,     8.954,    482.45,    643459,   132.719
Adjust Peak Width, Quad 1 - failed

--------------------------------------------------------------------------------
Adjust Mass Axis, Quad 1 - Starting - 4/19/2023 1:08:28 PM
      target low mass 69.00, target high mass 502.00, tolerance 1.00, adjustAB False
      axis offset, axis gain,    axis A,    axis B,  low mass, abundance, peakwidth, high mass, abundance, peakwidth
           -2.600,     19.88,      1.00,   0.00048,     69.78,  657049.3,     12.21,    524.33,  686829.1,     49.69
Adjust Mass Axis, Quad 1 - failed

--------------------------------------------------------------------------------
Adjust Peak Width, Quad 1 - Starting - 4/19/2023 1:08:31 PM
      target peak width 6.00, tolerance 2.00, adjustAB False
      axis offset, axis gain, pw offset,   pw gain,      pw A,      pw B,  low mass, abundance, peakwidth, high mass, abundance, peakwidth
           -0.600,     -5.12,   -1.3000,    -79.13,     -2.40,   0.00060,     64.88,    628196,    10.414,    523.72,    697160,    28.445
Adjust Peak Width, Quad 1 - failed

--------------------------------------------------------------------------------
Check Abundance, Quad 1 - Starting - 4/19/2023 1:08:33 PM
      Mass 69.00, Limits 2000000 to 6000000
             EMV,      mass, abundance, peak width
            3000,     73.22,     647908,      9.44
            3000,     72.83,     602247,      9.80
            3000,     71.23,     669577,      3.50
            3000,     67.79,     628847,     10.02
            3000,     67.08,     610131,      8.88
            3000,     71.47,     601765,      9.54
            3000,     73.65,     599042,     12.29
            3000,     70.79,     601895,     13.08
            3000,     74.00,     673450,     11.38
            3000,     67.87,     638831,     12.70
            3000,     69.07,     656465,     14.02
            3000,     67.64,     612510,      2.99
            3000,     69.38,     669817,      7.02
            3000,     71.00,     616264,      9.95
            3000,     71.21,     661775,      8.91
Check Abundance failed
Check Abundance, Quad 1 - Finished

--------------------------------------------------------------------------------
Adjust Mass Axis, Quad 1 - Starting - 4/19/2023 1:08:57 PM
      target low mass 69.00, target high mass 502.00, tolerance 6.00, adjustAB False
      axis offset, axis gain,    axis A,    axis B,  low mass, abundance, peakwidth, high mass, abundance, peakwidth
            1.400,    -30.12,      1.00,   0.00048,     72.00,  626382.9,      8.59,    504.20,  637716.3,      0.96
Adjust Mass Axis, Quad 1 - Finished

--------------------------------------------------------------------------------
Check Abundance, Quad 1 - Starting - 4/19/2023 1:09:00 PM
      Mass 69.00, Limits 2000000 to 6000000
             EMV,      mass, abundance, peak width
            3000,     71.66,     623318,      6.97
            3000,     67.39,     651209,     15.85
            3000,     72.77,     634542,    -78.06
            3000,     64.51,     633425,     10.68
            3000,     66.86,     633261,     10.25
            3000,     73.02,     664850,     26.57
            3000,     68.16,     652672,      6.18
            3000,     68.52,     619051,      7.82
            3000,     70.02,     620432,      9.97
            3000,     72.01,     647605,     10.56
            3000,     64.40,     672849,      9.69
            3000,     73.72,     593919,     10.99
            3000,     67.16,     671230,      9.48
            3000,     68.10,     627462,      6.24
            3000,     67.28,     658006,      2.65
Check Abundance failed
Check Abundance, Quad 1 - Finished

--------------------------------------------------------------------------------
Adjust Peak Width, Quad 1 - Starting - 4/19/2023 1:09:24 PM
      target peak width 2.00, tolerance 1.00, adjustAB True
      axis offset, axis gain, pw offset,   pw gain,      pw A,      pw B,  low mass, abundance, peakwidth, high mass, abundance, peakwidth
            1.400,    -30.12,   -0.6583,   -100.00,     -2.40,   0.00060,     69.98,    629123,    12.329,    483.38,    681675,     4.721
           -0.600,     -5.12,    1.3000,    -75.00,     -2.40,   0.00060,     71.28,    637958,    36.463,    490.57,    663562,    17.997
           -2.600,     19.88,    1.3000,    -50.00,     -4.80,   0.00060,     68.86,    586803,    10.687,    516.90,    659104,    50.508
           -0.600,     -5.12,    1.3000,    -75.00,     -4.80,   0.00120,     69.73,    664196,     9.314,    502.12,    698250,    42.615
           -1.429,     -8.95,    1.3000,   -100.00,     -4.80,   0.00060,     68.74,    729573,     9.654,    495.94,    632753,    51.439
           -2.094,     16.05,    1.3000,   -100.00,     -4.80,   0.00075,     69.97,    669154,    10.156,    520.99,    648322,    34.612
           -0.196,     -8.95,    1.3000,   -100.00,     -4.80,   0.00075,     67.38,    613448,     4.757,    518.50,    678198,    31.849
            1.804,    -33.95,    0.8666,   -100.00,     -4.80,   0.00075,     72.30,    671255,    10.888,    523.13,    690330,    17.491
            1.545,    -58.95,    1.3000,   -100.00,     -7.20,   0.00075,     66.66,    694267,     9.795,    487.68,    659324,    50.752
            1.976,    -33.95,    1.3000,   -100.00,    -10.80,   0.00075,     74.00,    646836,     9.269,    490.36,    677181,    49.726
           -0.024,     -8.95,    1.3000,   -100.00,    -10.80,   0.00075,     73.39,    696147,     6.663,    501.40,    731119,    21.714
           -2.024,     15.03,    1.3000,   -100.00,    -10.80,   0.00075,     68.48,    679967,    10.890,    510.15,    656808,    49.909
           -0.117,     -9.97,    1.3000,   -100.00,    -10.80,   0.00075,     70.87,    672787,    -3.674,    480.69,    655374,    48.728
           -2.117,     15.03,   -0.4314,   -100.00,    -10.80,   0.00075,     69.98,    628689,    11.014,    487.72,    672352,    37.869
           -2.600,     40.03,    0.0686,   -100.00,    -10.83,   0.00075,     69.59,    662151,     9.442,    501.66,    652651,    47.016
           -2.600,     44.53,    0.2502,   -100.00,     -6.56,   0.00075,     68.31,    628944,    10.003,    505.40,    684482,    48.150
           -1.114,     24.84,    0.5705,   -100.00,     -4.01,   0.00075,     71.97,    652455,     8.480,    487.20,    632725,    49.527
           -2.600,     49.84,    0.5605,   -100.00,     -6.20,   0.00075,     66.84,    675531,    10.073,    508.92,    707443,    20.356
           -0.600,     24.84,    1.2631,   -100.00,    -12.21,   0.00075,     71.74,    642254,     8.750,    500.31,    729095,    11.612
           -2.600,     46.16,    1.3000,   -100.00,     -4.58,   0.00075,     74.00,    675602,     8.935,    482.15,    693998,     7.277
Adjust Peak Width, Quad 1 - failed

--------------------------------------------------------------------------------
Adjust Peak Width failed

  • 0 in reply to TheoJooste

    When was the last time the ion source was cleaned?  Did you try filament 2?  New filaments?  Is there PFTBA in the vial?

    It is setting the EM voltage to 3000V, the maximum, to get even 600k abundance. You need abundance to get the system to go further. Resolve that first and the peak width might be OK.

  • 0 in reply to paulsalverda

    I have no idea when the source was last cleaned, it might have been a while ago (more than 1 year). Yes we tried filament 2 and the same error popped up. We checked the PFTBA vial and there are sufficient amounts left. 

    I will let my supervisor know tomorrow and then we might clean the source. I will also reply to this thread if the issue has been resolved.

    Thank you very much for your time and effort Paul, I really do appreciate it. 

  • 0 in reply to TheoJooste

    (1) Everything that comes out of your column goes into the mass spectrometer. Why you need to clean the ion source. - Blog - GC/MS - Agilent Community

    There are links to two videos that should help. The comprehensive one explains what to do. The HES one shows the unique parts of the HES.

    A year!  Many clean their ion source every other week or once a month or so.

  • 0 in reply to paulsalverda

    Thank you for the linked videos. They are very comprehensive and easily understood. We may try cleaning the source just to see if the problem still arises. 

    Yes, It might have been cleaned earlier than I mentioned however, due to the system being not part of our research group, it stands at a different part of the department with their own set rules and regulations. 

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