-------------------------------------------------------------------------------- Autotune - Starting - 4/19/2023 1:07:20 PM Software Revision MassHunter GC/MS Acquisition B.07.05.2479 PC Hostname DESKTOP-QRSMVU9 GCQQQ 7010B US1718W001 DSP: 7000.3509, qqqServer: G.7000.058-RUN Initial tune file D:\MassHunter\GCMS\1\7010\atunes.eihs.tune.xml -------------------------------------------------------------------------------- Tune parameters MS1DC 6.4 MS1PostFilterDC 6.4 MS1 Mass Axis Offset Dynamic enabled True 69, -1.774, 219, -1.737, 264, -1.762, 414, -1.786, 502, -1.779, MS1MassGain -0.42 MS1 Width Offset Dynamic enabled True 69, -0.015, 219, -0.002, 264, -0.005, 414, -0.008, 502, -0.013, MS1AMUGain 20.9 MS1Heater 150 MS2DC -4.6 MS2PreFilterDC -14.6 MS2 Mass Axis Offset Dynamic enabled True 69, -1.882, 219, -1.798, 264, -1.836, 414, -1.851, 502, -1.883, MS2MassGain 10.61 MS2 Width Offset Dynamic enabled True 69, -0.016, 219, -0.006, 264, -0.005, 414, -0.006, 502, -0.011, MS2AMUGain 13.7 MS2Heater 150 CCEntranceDC 7.4 CCHexapoleDC 6.4 CCHexapoleRFPeak 400 CCHexapoleDeltaV -5.0 CCExitDC 0.4 CCGasOn 1 Iris Dynamic enabled True 69, -3.5, 219, -12, 264, -16.5, 414, -26, 502, -30.5, 1050, -58.5, HED -10.0 EMV 1478 CollisionEnergy 0 SourceHeater 230 Repeller 16.9 IonBody 19.8 IonFocus -220.0 Entrance Lens Dynamic enabled True 69, -15.59995, 264, -16.99995, 414, -19.79996, 502, -21.99997, Filament 2 Emission 100.0 ElecEnergy 70 Extractor Dynamic enabled True 69, 14.60002, 264, 14.90002, 502, 14.60002, SensitivityMode 1 EinzelLens1 0.0 EinzelLens2 -12.0 GasSelect B GasFlow 0 FastScanOffset -4.0 H2Flow 0.00 -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Acquisition Settings MS1ScanMode = TTI MS1TTICutoff = 75 MS1SIMIon = 264 MS1ScanLowMass = 35 MS1ScanHighMass = 650 MS1ScanStep = 0.1 MS1ProfileMasses = 69.00, 10.00 MS1ProfileMasses = 264.00, 10.00 MS1ProfileMasses = 502.00, 10.00 MS2ScanMode = Profile MS2TTICutoff = 50 MS2SIMIon = 264 MS2ScanLowMass = 35 MS2ScanHighMass = 650 MS2ScanStep = 0.1 MS2ProfileMasses = 69.00, 10.00 MS2ProfileMasses = 264.00, 10.00 MS2ProfileMasses = 502.00, 10.00 ProfileSamples = 256 ProfileAverage = 1 ProfileStep = 0.1 ScanSamples = 16 ScanAverage = 3 ScanThreshold = 0 ScanIsCentroided = True SIMDwellTime = 20 CaptureSamples = 32000 CaptureDwellTime = 0.016 -------------------------------------------------------------------------------- Doing EI HES autotune Repeller set to 12 IonBody set to 10 IonFocus set to -180 Extractor set to 12 EntLens set to -20 MS1PostFilterDC set to 5 Iris set to -35 Lens 1 Offset set to -10 Lens 2 set to -25 Filament Select 2 -------------------------------------------------------------------------------- Find Peaks, Quad 1 - Starting - 4/19/2023 1:07:20 PM -------------------------------------------------------------------------------- Find Peaks mass 68.08, abundance 121.2, peak width 7.29 -------------------------------------------------------------------------------- Check Abundance, Quad 1 - Starting - 4/19/2023 1:07:29 PM Mass 69.00, Limits 100000 to 2000000 EMV, mass, abundance, peak width 1478, 66.86, 118, 9.86 1678, 72.01, 589, 8.65 1878, 69.13, 2728, 4.88 2078, 69.19, 9380, 9.62 2278, 68.98, 25904, 4.94 2478, 69.12, 71019, 11.93 2678, 72.31, 180491, 9.30 Check Abundance, Quad 1 - Finished Find Peaks mass 69.18, abundance 194208.2, peak width 8.52 Find Peaks mass 498.49, abundance 143799.7, peak width 10.79 Find Peaks mass 70.29, abundance 173434.9, peak width 8.86 Find Peaks mass 497.63, abundance 171518.2, peak width 50.83 -------------------------------------------------------------------------------- Check Abundance, Quad 1 - Starting - 4/19/2023 1:07:45 PM Mass 69.00, Limits 2000000 to 6000000 EMV, mass, abundance, peak width 2678, 69.09, 169955, 10.80 2878, 69.00, 426941, -16.58 3000, 69.17, 759604, 5.63 3000, 67.92, 614101, 1.69 3000, 69.04, 625889, 9.87 3000, 69.07, 748013, 7.78 3000, 68.89, 711960, 5.64 3000, 69.15, 601906, 10.55 3000, 68.98, 706757, 9.19 3000, 69.00, 729832, 9.49 3000, 69.19, 686079, 13.95 3000, 72.14, 604682, 11.96 3000, 72.70, 645750, 12.73 3000, 68.84, 689163, 8.97 3000, 68.97, 681005, 9.99 Check Abundance failed Check Abundance, Quad 1 - Finished -------------------------------------------------------------------------------- Adjust Mass Axis, Quad 1 - Starting - 4/19/2023 1:08:09 PM target low mass 69.00, target high mass 502.00, tolerance 1.00, adjustAB False axis offset, axis gain, axis A, axis B, low mass, abundance, peakwidth, high mass, abundance, peakwidth 0.000, 0.00, 1.00, 0.00048, 70.88, 709723.3, 10.66, 490.14, 672599.1, 11.51 Adjust Mass Axis, Quad 1 - failed -------------------------------------------------------------------------------- Adjust Peak Width, Quad 1 - Starting - 4/19/2023 1:08:13 PM target peak width 6.00, tolerance 2.00, adjustAB False axis offset, axis gain, pw offset, pw gain, pw A, pw B, low mass, abundance, peakwidth, high mass, abundance, peakwidth -2.000, 25.00, -0.0127, 20.87, -2.40, 0.00060, 69.54, 707630, 10.756, 520.63, 633053, 49.137 Adjust Peak Width, Quad 1 - failed -------------------------------------------------------------------------------- Adjust Mass Axis, Quad 1 - Starting - 4/19/2023 1:08:15 PM target low mass 69.00, target high mass 502.00, tolerance 1.00, adjustAB False axis offset, axis gain, axis A, axis B, low mass, abundance, peakwidth, high mass, abundance, peakwidth 0.000, 0.00, 1.00, 0.00048, 70.89, 773630.3, 20.76, 490.31, 635417.2, 49.89 Adjust Mass Axis, Quad 1 - failed -------------------------------------------------------------------------------- Adjust Peak Width, Quad 1 - Starting - 4/19/2023 1:08:17 PM target peak width 6.00, tolerance 2.00, adjustAB False axis offset, axis gain, pw offset, pw gain, pw A, pw B, low mass, abundance, peakwidth, high mass, abundance, peakwidth -2.000, 25.00, -0.5797, -4.13, -2.40, 0.00060, 69.60, 769411, 10.045, 499.80, 689953, 51.023 Adjust Peak Width, Quad 1 - failed -------------------------------------------------------------------------------- Adjust Mass Axis, Quad 1 - Starting - 4/19/2023 1:08:19 PM target low mass 69.00, target high mass 502.00, tolerance 1.00, adjustAB False axis offset, axis gain, axis A, axis B, low mass, abundance, peakwidth, high mass, abundance, peakwidth -2.600, 31.71, 1.00, 0.00048, 68.95, 916784.3, 4.22, 499.22, 629934.3, 33.04 Adjust Mass Axis, Quad 1 - failed -------------------------------------------------------------------------------- Adjust Peak Width, Quad 1 - Starting - 4/19/2023 1:08:22 PM target peak width 6.00, tolerance 2.00, adjustAB False axis offset, axis gain, pw offset, pw gain, pw A, pw B, low mass, abundance, peakwidth, high mass, abundance, peakwidth -2.600, 44.88, -1.3000, -29.13, -2.40, 0.00060, 69.88, 935183, 3.111, 515.38, 656675, 9.729 Adjust Peak Width, Quad 1 - failed -------------------------------------------------------------------------------- Adjust Mass Axis, Quad 1 - Starting - 4/19/2023 1:08:24 PM target low mass 69.00, target high mass 502.00, tolerance 1.00, adjustAB False axis offset, axis gain, axis A, axis B, low mass, abundance, peakwidth, high mass, abundance, peakwidth -1.492, 19.88, 1.00, 0.00048, 70.21, 659646.6, 8.76, 511.01, 684082.9, 41.76 Adjust Mass Axis, Quad 1 - failed -------------------------------------------------------------------------------- Adjust Peak Width, Quad 1 - Starting - 4/19/2023 1:08:26 PM target peak width 6.00, tolerance 2.00, adjustAB False axis offset, axis gain, pw offset, pw gain, pw A, pw B, low mass, abundance, peakwidth, high mass, abundance, peakwidth -1.465, -5.12, -1.3000, -54.13, -2.40, 0.00060, 72.97, 643000, 8.954, 482.45, 643459, 132.719 Adjust Peak Width, Quad 1 - failed -------------------------------------------------------------------------------- Adjust Mass Axis, Quad 1 - Starting - 4/19/2023 1:08:28 PM target low mass 69.00, target high mass 502.00, tolerance 1.00, adjustAB False axis offset, axis gain, axis A, axis B, low mass, abundance, peakwidth, high mass, abundance, peakwidth -2.600, 19.88, 1.00, 0.00048, 69.78, 657049.3, 12.21, 524.33, 686829.1, 49.69 Adjust Mass Axis, Quad 1 - failed -------------------------------------------------------------------------------- Adjust Peak Width, Quad 1 - Starting - 4/19/2023 1:08:31 PM target peak width 6.00, tolerance 2.00, adjustAB False axis offset, axis gain, pw offset, pw gain, pw A, pw B, low mass, abundance, peakwidth, high mass, abundance, peakwidth -0.600, -5.12, -1.3000, -79.13, -2.40, 0.00060, 64.88, 628196, 10.414, 523.72, 697160, 28.445 Adjust Peak Width, Quad 1 - failed -------------------------------------------------------------------------------- Check Abundance, Quad 1 - Starting - 4/19/2023 1:08:33 PM Mass 69.00, Limits 2000000 to 6000000 EMV, mass, abundance, peak width 3000, 73.22, 647908, 9.44 3000, 72.83, 602247, 9.80 3000, 71.23, 669577, 3.50 3000, 67.79, 628847, 10.02 3000, 67.08, 610131, 8.88 3000, 71.47, 601765, 9.54 3000, 73.65, 599042, 12.29 3000, 70.79, 601895, 13.08 3000, 74.00, 673450, 11.38 3000, 67.87, 638831, 12.70 3000, 69.07, 656465, 14.02 3000, 67.64, 612510, 2.99 3000, 69.38, 669817, 7.02 3000, 71.00, 616264, 9.95 3000, 71.21, 661775, 8.91 Check Abundance failed Check Abundance, Quad 1 - Finished -------------------------------------------------------------------------------- Adjust Mass Axis, Quad 1 - Starting - 4/19/2023 1:08:57 PM target low mass 69.00, target high mass 502.00, tolerance 6.00, adjustAB False axis offset, axis gain, axis A, axis B, low mass, abundance, peakwidth, high mass, abundance, peakwidth 1.400, -30.12, 1.00, 0.00048, 72.00, 626382.9, 8.59, 504.20, 637716.3, 0.96 Adjust Mass Axis, Quad 1 - Finished -------------------------------------------------------------------------------- Check Abundance, Quad 1 - Starting - 4/19/2023 1:09:00 PM Mass 69.00, Limits 2000000 to 6000000 EMV, mass, abundance, peak width 3000, 71.66, 623318, 6.97 3000, 67.39, 651209, 15.85 3000, 72.77, 634542, -78.06 3000, 64.51, 633425, 10.68 3000, 66.86, 633261, 10.25 3000, 73.02, 664850, 26.57 3000, 68.16, 652672, 6.18 3000, 68.52, 619051, 7.82 3000, 70.02, 620432, 9.97 3000, 72.01, 647605, 10.56 3000, 64.40, 672849, 9.69 3000, 73.72, 593919, 10.99 3000, 67.16, 671230, 9.48 3000, 68.10, 627462, 6.24 3000, 67.28, 658006, 2.65 Check Abundance failed Check Abundance, Quad 1 - Finished -------------------------------------------------------------------------------- Adjust Peak Width, Quad 1 - Starting - 4/19/2023 1:09:24 PM target peak width 2.00, tolerance 1.00, adjustAB True axis offset, axis gain, pw offset, pw gain, pw A, pw B, low mass, abundance, peakwidth, high mass, abundance, peakwidth 1.400, -30.12, -0.6583, -100.00, -2.40, 0.00060, 69.98, 629123, 12.329, 483.38, 681675, 4.721 -0.600, -5.12, 1.3000, -75.00, -2.40, 0.00060, 71.28, 637958, 36.463, 490.57, 663562, 17.997 -2.600, 19.88, 1.3000, -50.00, -4.80, 0.00060, 68.86, 586803, 10.687, 516.90, 659104, 50.508 -0.600, -5.12, 1.3000, -75.00, -4.80, 0.00120, 69.73, 664196, 9.314, 502.12, 698250, 42.615 -1.429, -8.95, 1.3000, -100.00, -4.80, 0.00060, 68.74, 729573, 9.654, 495.94, 632753, 51.439 -2.094, 16.05, 1.3000, -100.00, -4.80, 0.00075, 69.97, 669154, 10.156, 520.99, 648322, 34.612 -0.196, -8.95, 1.3000, -100.00, -4.80, 0.00075, 67.38, 613448, 4.757, 518.50, 678198, 31.849 1.804, -33.95, 0.8666, -100.00, -4.80, 0.00075, 72.30, 671255, 10.888, 523.13, 690330, 17.491 1.545, -58.95, 1.3000, -100.00, -7.20, 0.00075, 66.66, 694267, 9.795, 487.68, 659324, 50.752 1.976, -33.95, 1.3000, -100.00, -10.80, 0.00075, 74.00, 646836, 9.269, 490.36, 677181, 49.726 -0.024, -8.95, 1.3000, -100.00, -10.80, 0.00075, 73.39, 696147, 6.663, 501.40, 731119, 21.714 -2.024, 15.03, 1.3000, -100.00, -10.80, 0.00075, 68.48, 679967, 10.890, 510.15, 656808, 49.909 -0.117, -9.97, 1.3000, -100.00, -10.80, 0.00075, 70.87, 672787, -3.674, 480.69, 655374, 48.728 -2.117, 15.03, -0.4314, -100.00, -10.80, 0.00075, 69.98, 628689, 11.014, 487.72, 672352, 37.869 -2.600, 40.03, 0.0686, -100.00, -10.83, 0.00075, 69.59, 662151, 9.442, 501.66, 652651, 47.016 -2.600, 44.53, 0.2502, -100.00, -6.56, 0.00075, 68.31, 628944, 10.003, 505.40, 684482, 48.150 -1.114, 24.84, 0.5705, -100.00, -4.01, 0.00075, 71.97, 652455, 8.480, 487.20, 632725, 49.527 -2.600, 49.84, 0.5605, -100.00, -6.20, 0.00075, 66.84, 675531, 10.073, 508.92, 707443, 20.356 -0.600, 24.84, 1.2631, -100.00, -12.21, 0.00075, 71.74, 642254, 8.750, 500.31, 729095, 11.612 -2.600, 46.16, 1.3000, -100.00, -4.58, 0.00075, 74.00, 675602, 8.935, 482.15, 693998, 7.277 Adjust Peak Width, Quad 1 - failed -------------------------------------------------------------------------------- Adjust Peak Width failed