Reduced sensitivity with MeOH as a solvent

Hi Anatoly,

Do manual injection from syringe then see result.

If i believe that there is no leak and column is okay then problem could be due to Syringe / Septa /Jet..

You can trim the column from both end..

Thanks!  The column is new, and septum and liner were recently replaced.  I like the idea of trying a manual injection but I am honestly out of practice injecting manually so I'll probably introduce more error and will make results inconclusive.  Has no-one had a similar experience when changing from heptane to methanol as a solvent?  If not, it's probably a hardware error I would have to track down.

Thanks!  The column is new, and septum and liner were recently replaced.  I like the idea of trying a manual injection but I am honestly out of practice injecting manually so I'll probably introduce more error and will make results inconclusive.  Has no-one had a similar experience when changing from heptane to methanol as a solvent?  If not, it's probably a hardware error I would have to track down.

Any changes in procedure will force to go for optimization / fine tunning to get desire result and that could be consumables / hardware / method parameter change etc..

It's kind of research to find root cause..

Indeed!  And I am loving it .  I just need a lot of help and advice!

That is why, we are here to put comment / advice..

And for that I am grateful