Analyze Agilent Seahorse XF Real-Time ATP rate assay data using Agilent Seahorse Analytics
Analyze Agilent Seahorse XF Hu T Cell Activation Assay data using Agilent Seahorse Analytics software
Analyze Agilent Seahorse XF Substrate Oxidation Stress…
Hello APacana ,
The reason for the difference in response between the TIC and the EIC is because when you extract the TIC of a given precursor you are extracting the ion signal from all precursors that create a 72 product ion, using your example. If…
Hello AJohnson13 ,
Here is the PC setup guide for older Windows 10 versions of MassHunter. It includes the PC specifications.
Windows10_Setup.book (agilent.com)
The software installation instructions would be included as part of your original media…
At 1 mL/min pumping water, the pressures are as follows:
Pump + Column = 53 bar
Pump + Column + 0.1 mm ID 30 cm tubing = 70 bar
Pump + Column + Tubing + Flow Cell = 75 bar
I have also confirmed that this pressure fluctuation has nothing to do…
It all depends on your following steps. You can even use Methanol or Acetonitrile for the protein precipition: Protein Precipitation for Biological Fluid Samples Using Captiva EMR-Lipid
See also the chapter on protein precipitation in Agilent's Handbook…
The Digest of upcoming webinars has been updated as of April 30.
Did you miss April's GC Live Session Recording and Discussion Thread? Check it out here: ( +) Diagnostics From Your Desk, Troubleshooting From Your Kitchen Table - Follow-up Discussion…
Hello cjwwfd ,
B.06.00 is out of support at this point, but I can offer some general suggestions for current revisions and hopefully you can apply them to your version. Version 12.0 is the current release. If possible, I would investigate your options…
I'm experimenting the same error. Logbook displays <MSQQQ_1: QqQ fault detected: 1.23 vacuum system is not ready. This is typical during a pump down cycle or when the high vacuum pump is turned off during a vent cycle or fault condition>. Does anyone…
Hello DET ,
Sorry for the late response on this.
What sort of spiking do you see usually? What is the output of the FID, in pA?
Are you using any UPS systems, or are the GCs plugged directly into the wall?
This Information Applies To: 1290 Infinity (G4226x) and High Performance Autosamplers (G1367x)
Issue:
The Peristaltic Pump needs to be replaced if it has stopped working due to worn tubing, if it is leaking, or as part of annual maintenance of the...
This Information Applies To: Agilent Autosamplers G1313A, G1329A/B, G4226A, G1367A/B/C/D/E, G7129A/B/C.
Issue:
If during initialization or during a sequence, the "Metering Home Failed" error occurs, this article will guide through the procedure...
This Information Applies To: 1100, 1200 and 1260 Infinity I and 1260 Infinity II LC Pumps (Excluding preparative pumps with electromagnetic purge valve (EMPV): G1361A. Not applicable to any of the 1290 Infinity II modules with automatic purge valve: G4220X...
This Information Applies To: Agilent 1260 Infinity G1315x DAD or G1365x MWD Modules
Issue:
Removing and re-installing Visible and UV lamps in Agilent 1260 Infinity G1315x DAD or G1365x MWD Modules.
Both the visible and UV lamps are consumable...
This Information Applies To: Agilent 1260 Infinity Binary, Quaternary or Isocratic Pumps.
Issue:
Removal of the pump head from the pump metering drive for maintenance on an Agilent 1260 Infinity Binary, Quaternary or Isocratic Pump Module.
Steps...
This Information Applies To: All Agilent 1260 Infinity Binary, Quaternary, or Isocratic Pumps without the Seal Wash Option installed.
Issue:
Worn pump seals can shed small particles that can cause blockages and pressure issues downstream of the...
This Information Applies To: Agilent G7167x Multisampler modules
Issue:
Required when:
The needle seat is visibly damaged, blocked, or is leaking.
Leaks or blockages in the Vialsampler are observed, especially between the needle and needle...
This Information Applies To: Agilent 1260 Isocratic (G1310B) Quaternary (G1311B) Pumps.
Issue
Required when:
The inlet valve is defective.
The pressure or leak test in Lab Advisor suggests the inlet valve may require replacement.
A large...
This Information Applies To: Agilent 1260 Isocratic (G1310B) quaternary (G1311B) pump modules
Issue:
Required when:
The outlet valve is defective or leaking.
The pressure or leak test in Lab Advisor suggests the outlet valve may require replacement...
This Information Applies To: Agilent 1260 Isocratic (G1310B) quaternary (G1311B) pump modules
Issue:
The seal wear in procedure is important as it acts to "bed-in" the seals to ensure a leak free and pressure-tight pump head.
Required when:...