For Cell Mito stress test, Seahorse Cell Mito Stress Test Kit, the recommended working concentrations are 1.5 µM for Oligomycin and 0.5 µM for Rot/AA. The FCCP concentration needs to be optimized (0.5 µM-2 µM). The optimal final compound concentration…
In general, the %CV across a group and from plate to plate should both be < 20%. Higher CVs in this case was due to edge effect on outside of the plate.
GC/MS Photo Parts List for SQ and TQ sources
Stainless Steel, Inert, Extractor, HydroInert, Chemical Ionization, and the High Efficiency Source
These diagrams and parts lists are included in the Troubleshooting & Maintenance manual for the 5977 which…
The free Glycan Peak Assignment Tool was designed to provide additional confidence in identifying InstantPC or 2AB N-glycan peaks by predicted retention times of over 100 glycans. The new tool does not require users to upload sensitive data from N-glycan…
This Information Applies To: Agilent OpenLab CDS
Issue
Identifying the baseline codes and baseline code descriptions for OpenLab CDS
Background
OpenLab CDS Data Analysis includes the same integration algorithms as available in both OpenLAB...
This Information Applies To: Agilent OpenLab CDS
Issue
This article provides step-by-step instructions to filter a signal in an Intelligent Report.
Requirements
To be able to carry out the procedure as described, the privilege of Data Processing...
This Information Applies To: Agilent OpenLab CDS
Issue
This article outlines different ways to quantify the amount of compound in an unknown sample.
Background
Quantitation uses peak area or height to determine the amount of a compound in...
This Information Applies To: Agilent G1329A/B and G1313A Standard Autosampler
Issue
Replacing the needle seat of the Agilent 1100/1200/1260 Standard Autosampler may be required during regular maintenance, replacing a bent needle, or flow path blockage...
This Information Applies To: Agilent G1329A/B and G1313A Standard Autosampler
Issue
Replacing the needle of the Agilent 1100/1200/1260 Standard Autosampler may be required during regular maintenance, replacing a bent needle, or flow path blockage...
This Information Applies To: Agilent 1290 Infinity (G4226A) and Agilent 1260 Infinity (G1367E) HiP Autosamplers
Issue
Replacing the Needle Assembly of the Autosampler is required when:
The needle is visibly damaged.
Leaks or blockages in the...
This Information Applies To: Agilent 6000 Series LC/MS Systems equipped with MS40+ rotary vane pumps
Issue
Replacing the MS-40+ (rough pump) fluid periodically is a key maintenance procedure to improve vacuum performance and minimize the possibilities...
This Information Applies To: All Agilent 6000 Series LC/MS Systems without a gate valve for ion capillary installation/removal. For instructions for removing an ion capillary on LC/MS instruments with a gate valve installed, please click here
Issue...
This Information Applies To: Agilent 6000 series LC/MS instruments
Issue:
Cleaning the ion transfer capillary as part of regular maintenance or as part of troubleshooting when a loss of sensitivity in the instrument occurs.
Steps to follow ...
This Information Applies To: Agilent 6000 Series LC/MS systems.
Issue
Efficient electrospray ionization (ESI) in your LC/MS ion source requires a properly adjusted nebulizer needle in good condition. The nebulizer needle is a consumable item in...