On the GC, is the Not Ready LED lit? If so, press the Status button and the GC will tell you what is not ready.
Split or Splitless? If it's Splitless the GC may need to be set to AutoPrepRun if the CTC sampler is not configured to send the prep run…
We have not tested nor validated the use of Oligo Pro II data on ProSize so we cannot comment on why it would work for your colleagues. As for why only a single row is showing, please collect the zip file for your data as outlined in my previous message…
At 310° C ion source temperature, the 502 will always be lower than typical. Also - please reduce the quad temperature down to 150° or maybe as high as 165° C... High quad temperature reduces the lifetime of the quad and only very few applications need…
Hi Andreja,
For calculating area % of two different peaks you must set one baseline left and one baseline right from the peaks. Then you have to enable "Information Pane" when clicking "View" in the menu bar and the results are shown as given in the…
Hi,
Yes it is right, please orientate on the last peak before the impurities start.
The exact baseline settings can be seen when enabling the peak information section below the analysis view (highlighted in green):
Manual integration limits…
We were able to configure the plate, we are good for now.
The 1290 Infinity II we have is coupled to Sciex 6500 MS and is controlled by Analyst Software using the Analyst Device Driver.
This Information Applies To: Agilent Liquid Chromatography (LC) systems
Issue:
Shutting down the HPLC for a long period requires following this general procedure to prevent damage to the system and the column.
Steps to follow:
Store the...
This Information Applies To: Agilent G7129A/B Autosampler with Sample Thermostat/Cooler G7167-60101 or G7167-60005.
Issue:
The autosampler may stop working from time to time, potentially giving off loud noises and switching off the light. The only...
This Information Applies To: Agilent 5977A/B GC/MSD with EI source, EI Extractor Source, or CI Source.
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte...
This Information Applies To: Agilent HES source, Agilent 5977 Series GC/MSD
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte response not improving...
This Information Applies To: Agilent 5977 and 5975 Series GC/MSD systems
Issue
The autotune fails and reports the error Tune action stopped: Gain and offset = 0 and peaks are too narrow!
Resolution
The MSD autotune program is run after routine...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
Condition new or cleaned ICP-MS skimmer cones and sampler cones by aspirating a Conditioning Solution before use.
Background
Drift up can be a sign of...
Turn On the GC/MS Interface and Oven Using OpenLab CDS
Multiple Results
This Information Applies To: Agilent GC/MS instruments controlled by OpenLab CDS software
Issue
Turning on the GC/MS interface heater and Oven using OpenLab CDS software.
Steps to Follow
From the Home tab, click Method > Instrument Setup...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900)
Issue
The signal monitor function in MassHunter can help with troubleshooting low sensitivity, carry over, contamination, or high background. It can also be used to optimize...
This Information Applies To: Agilent UV detectors (Variable Wavelength Detectors, Multiple Wavelength Detectors, and Diode Array Detectors)
Issue
Checking accumulated hours on the UV lamp.
Steps to Follow
There are four ways to check the accumulated...
This Information Applies To: Agilent 7700/7800/7850/7900/8800/8900 ICP-MS
Issue:
Poor internal standard (ISTD) mixing can cause high relative standard deviation (RSD).
The recommendations in this article are applicable if:
It takes a long...