This Information Applies To: Agilent HPLC systems
The presence of ghost peaks can be a symptom of a system contamination issue, caused by accumulation of sample and/or foreign substances in the flow path. One common source of contamination and carry-over of peaks from previous sample runs is the LC column.
In addition to the presence of ghost peaks, some increase in pressure may have been noticed. This article focuses on troubleshooting a contamination issue that is suspected to originate from the column.
The following parts may be required during the troubleshooting process:
Steps to Follow
|Confirmation: A blank run is a run with only injecting the solvent that samples are dissolved in.
Caution: Always check your column suppliers recommendations for a safe back-flushing procedure, and check what are the safe solvents to use for your specific column and application. Use of incompatible solvents or excess pressure can cause column damage
If you cannot locate the source of ghost peaks after working through these steps, contact your local Agilent Support representative for further technical support.
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