Manual integration of Qualifier is not working. Mass hunter v10.

Question

Hello, 
 I am trying to quantitate a radioactive compound. I have the unlabeled standard and it sets up a cal curve very well. However, the synthetic samples suffer from degradation and it generates two other compounds in addition to parent analyte. 
 So I am trying to use analyte as quantifier, with the two degradants as qualifiers along with the area sum feature. 
 But when I analyze batch only one of the qualifiers integrates and adds to the calculated concentration. Occasionally, I can use the split peak and pick right to switch from one qualifier to the other but I can only ever get one of the two to integrate and be part of the compound table calculations.

The middle qualifier is the one that will not integrate even though the signal strength is greater. 
 Thanks for the help 
 Scott

howard_sanford · Accepted Answer

Hello ScottAtJNJ , 
 I would make certain that your Correlation Window is set wide enough to include this ion as a qualifier. This setting is found in the Globals Setup section of the method and is given in minutes. 
 
 If you are still having trouble getting it to integrate you could try opening up the RT Delta windows to include more baseline for the integrator to use for peak detection, or try using another integrator, if that is allowed for your method. 
 Another option to consider, if you are not utilizing qualifier outliers for this ion, would be to set it up as a separate compound and use compound groups and math to create an aggregate compound to evaluate. Here is a link to a document with details on this feature. 
 Compound-Groups-Quant-B.07.01.pdf (agilent.com)

Manual integration of Qualifier is not working. Mass hunter v10.

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