Is it possible to programmatically create LCMS acquisition methods starting from a base acquisition method?

megibb

I am not sure about the answer for your question.  But you can specify MS parameters in the Worklist that override the method settings and this may help you out.

megibb

I am not sure about the answer for your question.  But you can specify MS parameters in the Worklist that override the method settings and this may help you out.

Thanks for your suggestion. Can I vary source parameters and CE in this way?

Matt

It looks like these parameters can be overridden-

Including source conditions and CE,

Thanks Andy! This is great. I don't see how to do this in MH12 though -is this still possible in MH12?

My apologies for an extra post, but I do not see how to override CE values in the worklist. It would be amazing if I can do this, but I don’t see how to do this in Mass Hunter.

Is this for a QQQ or a QToF?  What version of MH Acquistion?   If it is MH Acquisition version 12, then it will be a QQQ.   For the QQQ, different CEs cannot be set in the worklist and instead you may want to try a range of CEs using the Optimizer option or including a series of CEs in your transition settings in a method.

Yes -my apologies: this is Mass Hunter 12 on a 6495C QQQ.

My situation was that I needed to transfer a method with 143 analytes (over 400 MRMs) from a Sciex to our new 6495C. The on board source optimization tools in Mass Hunter turned out to be amazing (!!) but we needed an efficient way to manage all of the MRM CE optimizations, sticking to the transitions defined in the existing method.

It turned out that the most efficient way to do this was to create a base method and then modify the base method to create multiple CE methods and then acquire a sequence. The areas were then obtained from MH Qual and some custom code was written to select the best CE for each MRM transition.

Again, my apologies for not providing enough information in my question.