Good morning, everybody!
I am currently doing untargeted analysis using the Unknown analysis software. I could «identify» some compounds of interest, which is great. Now, my next step is to do a semi-quantification of my compounds using my internal standard. I have the option to use the FID signal or TIC, but I do have co-elution. Therefore, I started using the component area, which is (I believe) more specific to a compound and could take care of the coelution problem.
My question is: Can I use the component area of the identified compounds, including my internal standard and manually calculate the ratio (Area,cp/Area,Std*C,Std) for semi-quantification?
I would appreciate, before going further to have your opinion about this.