Missing qTOF Reference Ions

Hello,

 

I am not seeing all of the reference ions (negative or positive) while my qTOF instrument is in idle or acquisition.  Spectrum and Work List icons remain yellow whereas Vial icon remains red. A fresh reference solution was prepared, flow and function of nebulizer confirmed. Some additional details as follows:

 

  • In negative mode, seeing only 112 and 1033 m/z.  Not seeing 119, 301, 966, 980, 1633 m/z.  Similar scenario in positive mode; seeing only 121 and 922 m/z.
  • Played with MP flow rate, reference nebulizer pressure and other parameters to no avail.
  • I have a dual ESI source.  Set up for small molecule analysis; 1700 m/z, 2 GHz.
  • Instrument recently passed calibration and tune for TOF and Quad.
  • Acquisition software recently upgraded to Version 10.  Methinks this is the culprit..?

 

Best regards,

Mark

Parents
  • Agilent service rep came out a second time and was able to clear up issue (cleaning of ref nebulizer and associated lines).  After running my method (gradient of 0.1%HOAc MeOH into H2O), ions (112 and 119) lost; indicators changing from green to yellow (same as observed on Dec 27).  Methinks the fixed 10 psi used to deliver the ref ion solution is to low.  From what I gather, others may have similar issues.

Reply
  • Agilent service rep came out a second time and was able to clear up issue (cleaning of ref nebulizer and associated lines).  After running my method (gradient of 0.1%HOAc MeOH into H2O), ions (112 and 119) lost; indicators changing from green to yellow (same as observed on Dec 27).  Methinks the fixed 10 psi used to deliver the ref ion solution is to low.  From what I gather, others may have similar issues.

Children
No Data
Was this helpful?