Hello, could you advise me? JetStream ESI Agilent 6470a coupled with Agilent 1260 Infiniti.
The signal is completely gone. It doesn't matter where the liquid comes from in mass-spec - from HPLC, through the tuning line or directly from the syringe - the effect is the same - zero signal. Tuning mixture, chloramphenicol, reserpine, polytyrosine - nothing is detected.
At the same time, the nebulizer forms a good spray. What could be the problem?
P.S. It is interesting that the capillary current does not change much and, regardless of the presence or absence of a spray, it remains about 2000 nA. Could this be an isolator problem?
