Hi
Comparing my tune signal now and months and years ago, I see a drop in signal. It used to be in the 300.000 for low mass and 50K for high. Now I have 50K for low and very low (~1000) for high. Last time only 290 for the high mass (see report attached). So the Tune passes, but I must lose sensitivity.
I cleaned the capillary, and it did not help too much.
How should I proceed?
- clean ion funnel?
- clean optics?
- get a new capillary?
Any advice?
Thank you
Dear Andy
Thank you for your reply.
I run also autotune and I got similar results.
What would be the best way to troubleshoot this issue?
1. I will replace the Tune Mix and check the tune again.
2. If this does not fix it, then I assume I have to clean ion optics assembly?
3. If this does not fix it, clean the high-energy dynode.
4. If this does not fix it, replace the electron multiplier horn
From the manual (G1960-90154_SingleQuad_Maintenance.pdf), it is not clear where the electron multiplier is located. Are there better instructions I can follow?
They only provide two images, without context on how to reach it:
Dear Andy
Thank you for your reply.
I run also autotune and I got similar results.
What would be the best way to troubleshoot this issue?
1. I will replace the Tune Mix and check the tune again.
2. If this does not fix it, then I assume I have to clean ion optics assembly?
3. If this does not fix it, clean the high-energy dynode.
4. If this does not fix it, replace the electron multiplier horn
From the manual (G1960-90154_SingleQuad_Maintenance.pdf), it is not clear where the electron multiplier is located. Are there better instructions I can follow?
They only provide two images, without context on how to reach it:
Hi Andy
Thanks for your help. From what you said, and looking at the autotune files, it looks the EM is almost dead.
I spoke to Agilent and the difference in m/z detection suggested them that the optics are dirty.
I am attaching a comparison between autotune 2014 and from January. 
Thanks
Daniel
