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I am using an InfinityLab Poroshell 120 HILIC-Z. For pH adjustment, 0.2N HCl. Source conditions (if I understand what you mean) are ESI with a flow rate of 0.5mL/min.
Hi Katy,
Looks like you are coating the spray chamber with ammonium chloride, not ammonium format. I wouldn't recommend using HCl to reduce the pH of your ammonium formate. Adjust the pH with formic acid instead, and he problem should go away.
Kind regards
Andy
Hi Katy,
Looks like you are coating the spray chamber with ammonium chloride, not ammonium format. I wouldn't recommend using HCl to reduce the pH of your ammonium formate. Adjust the pH with formic acid instead, and he problem should go away.
Kind regards
Andy
I think Andy's right (pun intended). I've seen this a couple times before where insoluble salts were formed when adjusting pH.
I would probably recommend having your system cleaned/PM'd if you are able to. Last time this happened, it made it all the way back to the quads.