Problem in LCMS G6125B

Hi

Remove column and then run ACN to check that still peak is coming..

U can use union to connect lines..

I tried to inject ACN with two different columns, and I get about seven unknown peaks for run time 5 min, I think something contamination in MS, but How to confirm this error from MS?

HI sherifv4

They look like chromatographic peaks, rather than contamination of the MS.  Can you share the spectra from each of those peaks?

/Andy

Is this require cleaning ?, And s there cleaning procedure for ion source block and capillary?

Check with this manual...

Should be like this..

Hello, Wenn die Verunreinigungen als Peaks mit Retentionzeit auftauchen dann kann die Verunreinigung nicht vom Massenspelktrometer sprich Quelle kommen sonder vom LC mit allen Möglichkeiten wie verschmutztes Fliesmittel (Wasser oder Organik), verdreckter Autosampler durch ehemalige Proben usw.

Hello, If the impurities appear as peaks with retention time, then the impurities cannot come from the mass spectrometer, i.e. the source, but from the LC with all possibilities such as contaminated fluid (water or organic), dirty autosampler from previous samples, etc.

Above possibleties, i already mentioned in starting of my replies to check hplc module/columns via bypassing with union to confirm that problem is with ms or hplc..

Looks like that could do with a bit of a clean!

https://www.agilent.com/en/video/cleaning-electrospray-spray-chamber-weekly

Andy

Looks like that could do with a bit of a clean!

https://www.agilent.com/en/video/cleaning-electrospray-spray-chamber-weekly

Andy