# LC-MS TOF 6230

Hello all,

I have a problem with trying to tune/calibrate the TOF. When I try to turn on my calibrant, I see the 6 ions briefly but after a few seconds they disappear, also the ions 120 and 922 appear to get intense overtime and gets very high and then all the ions disappear even though the calibrant is on.

I tried turning the reference mass and I see the 120 and 922 ions consistently for however long I leave it with no issues.

Any guidance in this would be much appreciated, thanks!