Signal drops during runs

Hello all,

We have having problems with our LC/MS with the TIC signal dropping below the baseline at various points during many runs (see image), including in "blank" samples/washes (both positive and negative mode).

Our setup: 1290 Infinity II LC, diamond hydride silica HPLC column (solvents H2O and acetonitrile, 0.2% acetic acid), with 6545 QTOF (detecting m/z range 50-1200).

We have tried a new column, different batches of solvent, and re-tuning but no luck...

Help??

(Please excuse my inexperience, I am new to this world).

Thanks,

Will

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0 tmclaren over 2 years ago

Makes sure that the spray for the reference nebulizer is not sputtering when introducing reference stock.
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0 Andy Wright over 2 years ago in reply to tmclaren

.I would also check the spray from the main nebulizer, to see if this is sputtering too. Also, check the wide exhaust tube from the spray chamber and the waste bottle that it is connected to. Is the flow obstructed by liquid or some other blockage?

/Andy
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0 Andy Wright over 2 years ago in reply to tmclaren

.I would also check the spray from the main nebulizer, to see if this is sputtering too. Also, check the wide exhaust tube from the spray chamber and the waste bottle that it is connected to. Is the flow obstructed by liquid or some other blockage?

/Andy
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